Recombinant Mouse CRELD2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 3686-CR

R&D Systems, part of Bio-Techne
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3686-CR-050
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Key Product Details

Source

NS0

Accession #

Q9CYA0

Conjugate

Unconjugated

Applications

Bioactivity

View all CRELD2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CRELD2 protein
Ser23-Leu350, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser23

Predicted Molecular Mass

36.8 kDa

SDS-PAGE

57-62 kDa, reducing conditions

Activity

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells.
The ED50 for this effect is typically 0.75-3 μg/mL.

Formulation, Preparation and Storage

3686-CR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CRELD2

Cysteine‑rich with EGF‑like domain protein 2 (CRELD2) is an approximately 60 kDa glycoprotein that contains two EGF‑like domains and two FU domains (1). Mature mouse CRELD2 shares approximately 77% and 92% aa sequence identity with human and rat CRELD2, respectively. It is widely expressed in fetal and adult tissues (1, 2). CRELD2 localizes to the endoplasmic reticulum and Golgi and can also be secreted (2‑4). It is up‑regulated during the cellular stress‑induced unfolded protein response (UPR), cartilage and bone pathologies (MED and MCDS), and arsenic‑induced liver toxicity (3, 5‑7). CRELD2 interacts intracellularly with the alpha4 and beta2 subunits of the nicotinic acetylcholine receptor and inhibits cell surface expression of the receptor (2, 8). CRELD2 expression is up‑regulated in prostate cancer by dihydroxytestosterone and is down‑regulated following anti‑androgen treatment (9).

References

  1. Rupp, P.A. et al. (2002) Gene 293:47.
  2. Ortiz, J.A. et al. (2005) J. Neurochem. 95:1585.
  3. Oh-hashi, K. et al. (2009) Biochem. Biophys. Res. Commun. 387:504.
  4. Oh-hashi, K. et al. (2011) FEBS Lett. 585:2481.
  5. Cameron, T.L. et al. (2011) PLoS ONE 6:e24600.
  6. Nundlall, S. et al. (2010) Cell Stress Chaperones 15:835.
  7. Nohara, K. et al. (2012) Toxicol. Sci. epub.
  8. Hosur, V. et al. (2009) J. Neurochem. 111:848.
  9. Jariwala, U. et al. (2007) Mol. Cancer 6:39.

Long Name

Cysteine-rich Protein With EGF-like Domains 2

Alternate Names

cysteine-rich with EGF-like domain protein 2, cysteine-rich with EGF-like domains 2, DKFZp667O055, MGC11256

Entrez Gene IDs

79174 (Human); 76737 (Mouse)

Gene Symbol

CRELD2

UniProt

Q9CYA0 (Mouse)

Additional CRELD2 Products

Product Documents for Recombinant Mouse CRELD2 Protein, CF

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Product Specific Notices for Recombinant Mouse CRELD2 Protein, CF

For research use only

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