Recombinant Mouse CRISP-1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 4675-CR

R&D Systems, part of Bio-Techne
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4675-CR-050
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Key Product Details

Source

NS0

Accession #

Q03401

Conjugate

Unconjugated

Applications

Bioactivity

View all CRISP-1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CRISP-1 protein
Gln20-His244, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln20 predicted: No results obtained, sequencing might be blocked.

Predicted Molecular Mass

26.4 kDa

SDS-PAGE

30 kDa, reducing conditions.

Activity

Measured by its ability to inhibit proliferation/survival of the 3A-sub E human placenta cell line.
The ED50 for this effect is 0.12-0.6 μg/mL.

Formulation, Preparation and Storage

4675-CR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CRISP-1

Cysteine‑rich secretory protein 1 (CRISP‑1), also known as Acidic Epididymal Glycoprotein 1 (AEG1) and Sperm‑Coating Glycoprotein 1 (SCP1), is a 32 kDa protein that is involved in male reproductive biology (1, 2). CRISPs belong to the CAP superfamily of molecules that also includes several snake, insect, and lizard venom proteins (3). Structurally, CRISPs consist of an N‑terminal SCP/CAP domain, a hinge region, and a Cys‑rich domain with 16 invariant cysteine residues (4). CRISPs are expressed in discrete but overlapping regions of the male reproductive tract and play a role in spermatozoa adhesion with Sertoli cells and oocytes, decapacitation, and the acrosomal reaction (1). Expression patterns, genomic structure, and sequence conservation indicate that the likely ortholog of human CRISP‑1 in mouse and rat is CRISP‑4 (5, 6). Mature mouse CRISP‑1 shares 68% amino acid (aa) sequence identity with rat CRISP‑1. It shares 55%, 73%, and 43% aa sequence identity with mouse CRISP‑2, ‑3, and ‑4, respectively. Rodent CRISP‑1 is secreted by the epididymal epithelium and associates with the surface of spermatozoa in the epididymis where it inhibits capacitation (7 ‑ 9). It is released from the sperm cell surface once capacitation begins in the female reproductive tract or in vitro (10). CRISP‑1 is involved in the interaction of spermatozoa with the oocyte zona pellucida as well as in the fusion of sperm and egg (11, 12). CRISP‑1 is additionally expressed in the lower medulla of hair shafts and under androgen control in the submandibular salivary glands of male mice (13 ‑ 15).

References

  1. Koppers, A.J. et al. (2011) Asian J. Androl. 13:111.
  2. Gibbs, G.M. et al. (2008) Endocr. Rev. 29:865.
  3. Yamazaki, Y. and T. Morita (2004) Toxicon 44:227.
  4. Guo, M. et al. (2005) J. Biol. Chem. 280:12405.
  5. Jalkanen, J. et al. (2005) Biol. Reprod. 72:1268.
  6. Nolan, M.A. et al. (2006) Biol. Reprod. 74:984.
  7. Charest, N.J. et al. (1989) Endocrinology 125:942.
  8. Maldera, J.A. et al. (2011) Biol. Reprod. 85:503.
  9. Nixon, B. et al. (2006) Biol. Reprod. 74:275.
  10. Roberts, K.P. et al. (2003) Biol. Reprod. 69:572.
  11. Busso, D. et al. (2007) Biol. Reprod. 77:848.
  12. Da Ros, V.G. et al. (2008) Dev. Biol. 320:12.
  13. Haendler, B. et al. (1993) Endocrinology 133:192.
  14. Mizuki, N. and M. Kasahara (1992) Mol. Cell. Endocrinol. 89:25.
  15. Peterson, R.L. et al. (2005) J. Investig. Dermatol. Symp. Proc. 10:238.

Long Name

Cysteine-rich Secretory Protein 1

Alternate Names

AEGL1, CRISP1

Entrez Gene IDs

167 (Human); 11571 (Mouse); 64827 (Rat)

Gene Symbol

CRISP1

UniProt

Q03401

Additional CRISP-1 Products

Product Documents for Recombinant Mouse CRISP-1 Protein, CF

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Product Specific Notices for Recombinant Mouse CRISP-1 Protein, CF

For research use only

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