Recombinant Mouse CRISP-2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 4576-CR

R&D Systems, part of Bio-Techne
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4576-CR-050
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Key Product Details

Source

NS0

Accession #

P16563

Conjugate

Unconjugated

Applications

Bioactivity

View all CRISP-2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CRISP-2 protein
Lys23-His243 with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys23

Predicted Molecular Mass

25.9 kDa

SDS-PAGE

27 kDa, reducing conditions

Activity

Measured by its ability to inhibit proliferation/survival of the 3A-sub E human placenta cell line.
The ED50 for this effect is 0.5-2.5 μg/mL.

Formulation, Preparation and Storage

4576-CR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CRISP-2

CRISP-2, also known as Tpx-1, is one of several CRISPs (cysteine-rich secretory proteins) that contain 16 invariant cysteine residues and show structural homology to snake, insect, and lizard venom proteins. These proteins consist of an N-terminal SCP/CAP domain, a hinge region, and a cysteine-rich CRISP domain. CRISPs are expressed in discrete but overlapping regions of the male reproductive tract and play a role in spermatozoa adhesion with Sertoli cells and oocytes, decapacitation, and the acrosome reaction (1, 2). Mature mouse CRISP‑2 shares 73% and 86% aa sequence identity with human and rat CRISP-2, respectively (3). CRISP-2 is expressed in late pachytene spermatocytes, round and elongated spermatids, and mature spermatozoa (4, 5). It is found in cytoplasmic granulated bodies and in association with Gametogenetin 1, SHTAP, outer dense fibers of the tail, and the acrosome (5-9). CRISP-2 is involved in spermatocyte interactions with Sertoli cells as well as in sperm-egg fusion (7, 8, 10). It regulates calcium flux during sperm capacitation by increasing or decreasing the open time of the ryanodine receptors RyR1 and RyR2, respectively (11).

References

  1. Koppers, A.J. et al. (2011) Asian J. Androl. 13:111.
  2. Gibbs, G.M. et al. (2008) Endocr. Rev. 29:865.
  3. Kasahara, M. et al. (1989) Genomics 5:527.
  4. O’Bryan, M.K. et al. (1998) Mol. Reprod. Dev. 50:313.
  5. O’Bryan, M.K. et al. (2001) Mol. Reprod. Dev. 58:116.
  6. Jamsai, D. et al. (2008) Reproduction 135:751.
  7. Busso, D. et al. (2007) Biol. Reprod. 76:701.
  8. Busso, D. et al. (2005) Mol. Hum. Reprod. 11:299.
  9. Jamsai, D. et al. (2010) Biol. Cell 102:93.
  10. Maeda, T. et al. (1998) Biochem. Biophys. Res. Commun. 248:140.
  11. Gibbs, G.M. et al. (2006) J. Biol. Chem. 281:4156.

Long Name

Cysteine-rich Secretory Protein 2

Alternate Names

CRISP2, GAPDL5, TPX1, TSP1

Entrez Gene IDs

7180 (Human); 22024 (Mouse)

Gene Symbol

CRISP2

UniProt

P16563

Additional CRISP-2 Products

Product Documents for Recombinant Mouse CRISP-2 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Mouse CRISP-2 Protein, CF

For research use only

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