Recombinant Mouse CRISP-4 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5017-CR

R&D Systems, part of Bio-Techne
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5017-CR-050
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Key Product Details

Source

CHO

Accession #

NP_084309

Conjugate

Unconjugated

Applications

Bioactivity

View all CRISP-4 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse CRISP-4 protein
Pro22-Lys250, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu23

Predicted Molecular Mass

27.2 kDa

SDS-PAGE

34-37 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of NTera-2 human testicular embryonic carcinoma cells.

The ED50 for this effect is 0.3-1.5 μg/mL after a 1 hour incubation at
37 °C.

Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

5017-CR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CRISP-4

CRISP-4 is one of several CRISPs (cysteine-rich secretory proteins) that contain 16 invariant cysteine residues and show structural homolgy to snake, insect, and lizard venom proteins. These proteins consist of an N-terminal SCP/CAP domain, a hinge region, and a cysteine-rich CRISP domain. CRISPs are expressed in discrete but overlapping regions of the male reproductive tract and play a role in spermatozoa adhesion with Sertoli cells and oocytes, decapacitation, and the acrosomal reaction (1 - 3). CRISP-4 facilitates the progesterone-induced acrosomal reaction by inhibiting calcium conductance through the TRPM8 channel on sperm (4). Mouse CRISP-4 is secreted as a 32 kDa protein in the epididymis (primarily the caput region), vas deferens, and seminal vesicle (5, 6). It is also associated with spermatozoa from the testis and caudal epididymis as a 26 - 28 kDa molecule (6). Its expression is induced at the onset of puberty in an androgen-dependent fashion (5). Mouse and rat CRISP-1 are additionally expressed in salivary glands, while CRISP-4 is not (6). Expression patterns, genomic structure, and sequence conservation indicate that CRISP-4 in mouse and rat are the likely orthologs of human CRISP-1 (5, 6). Mature mouse CRISP-4 shares 90% and 64% aa sequence identity with rat CRISP-4 and human CRISP-1, respectively (5). Mature mouse CRISP-4 shares 38% - 43% aa sequence identity with mouse CRISP-1, -2, and -3.

References

  1. Koppers, A.J. et al. (2011) Asian J. Androl. 13:111.
  2. Gibbs, G.M. et al. (2008) Endocr. Rev. 29:865.
  3. Maeda, T. et al. (1998) Biochem. Biophys. Res. Commun. 248:140.
  4. Gibbs, G.M. et al. (2011) Proc. Natl. Acad. Sci. 108:7034.
  5. Jalkanen, J. et al. (2005) Biol. Reprod. 72:1268.
  6. Nolan, M.A. et al. (2006) Biol. Reprod. 74:984.

Long Name

Cysteine-rich Secretory Protein 4

Alternate Names

CRISP4

Entrez Gene IDs

78081 (Mouse); 654517 (Rat)

Gene Symbol

CRISP4

UniProt

NP_084309

Additional CRISP-4 Products

Product Documents for Recombinant Mouse CRISP-4 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse CRISP-4 Protein, CF

For research use only

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