Recombinant Mouse CTRP4/C1qTNF4 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 2137-TN

R&D Systems, part of Bio-Techne
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2137-TN-050
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Key Product Details

Source

NS0

Accession #

Q8R066

Conjugate

Unconjugated

Applications

Bioactivity

View all CTRP4/C1qTNF4 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CTRP4/C1qTNF4 protein
Leu17-Leu326, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu17

Predicted Molecular Mass

34 kDa

SDS-PAGE

34-38 kDa, reducing conditions

Activity

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Recombinant Mouse CTRP4/C1qTNF4, immobilized at 2.5-5 μg/mL on a 96-well plate, is able to significantly enhance neurite outgrowth.

Scientific Data Images for Recombinant Mouse CTRP4/C1qTNF4 His-tag Protein, CF

Recombinant Mouse CTRP4/C1qTNF4 His-tag Protein SDS-PAGE

Recombinant Mouse CTRP4/C1qTNF4 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Mouse CTRP4/C1qTNF4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 34-38 kDa.

Formulation, Preparation and Storage

2137-TN
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 2 weeks, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: CTRP4/C1qTNF4

C1qTNF4, also known as CTRP4, is an approximately 34 kDa member of the C1q family of secreted proteins (1, 2). C1qTNF4 consists of a signal peptide followed by two tandem globular C1q domains (1). It is the only C1q family member with tandem C1q domains (1, 2). The second C1q domain contains an RGD peptide, suggesting that it could potentially interact with integrins (3). Mature mouse C1qTNF4 shares 95% and 98% amino acid sequence identity with human and rat C1qTNF4, respectively. Expression of human C1qTNF4 occurs most abundantly in brain and adipose tissue (1). Neurons in the hypothalamus express and secrete CTRP4 which modulates food intake and energy balance by controlling neuropeptide gene expression (1). In-house finding shows CTRP4 can support neurite outgrowth of cortical neurons. Like other CTRP family members, C1qTNF4 can form dimers, trimers, hexamers, and high molecular weight oligomers following secretion (1, 4). Injection of recombinant C1qTNF4 into mice can reduce food intake, body weight, and ambulatory activity levels (1). C1qTNF4 has also been shown to promote human cancer cell survival in vitro (5).

References

  1. Byerly, M.S. et al. (2014) J. Biol. Chem. 289:4055.
  2. Kishore, U. et al. (2004) Trends Immunol. 25:551.
  3. Ruoslahti, E. (1996) Annu. Rev. Cell Dev. Biol. 12:697.
  4. Wong, G.W. et al. (2008) Biochem. J. 416:161.
  5. Li, Q. et al. (2011) Cancer Lett. 308:203.

Long Name

C1q And Tumor Necrosis Factor Related Protein 4

Alternate Names

C1qTNF4, ZACRP4

Entrez Gene IDs

114900 (Human); 67445 (Mouse); 311184 (Rat)

Gene Symbol

C1QTNF4

UniProt

Q8R066

Additional CTRP4/C1qTNF4 Products

Product Documents for Recombinant Mouse CTRP4/C1qTNF4 His-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse CTRP4/C1qTNF4 His-tag Protein, CF

For research use only

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