Recombinant Mouse CTRP9/C1qTNF9 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 2132-TN

R&D Systems, part of Bio-Techne
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2132-TN-050
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Key Product Details

Source

NS0

Accession #

Q4ZJN1

Conjugate

Unconjugated

Applications

Bioactivity

View all CTRP9/C1qTNF9 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CTRP9/C1qTNF9 protein
Mouse C1qTNF9
(Gln20-Ser333)
Accession # Q4ZJN1		 HPGGGSGGGSGGGS HHHHHH
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln20 inferred from enzymatic pyroglutamate treatment revealing Asp21.

Predicted Molecular Mass

34 kDa

SDS-PAGE

39-43 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse CTRP9/C1qTNF9 is immobilized at 0.5 µg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human CL-K1/COLEC11 that produces 50% of the optimal binding response is 15-90 ng/mL.

Scientific Data Images for Recombinant Mouse CTRP9/C1qTNF9 His-tag Protein, CF

Recombinant Mouse CTRP9/C1qTNF9 His-tag Protein Binding Activity

Recombinant Mouse CTRP9/C1qTNF9 His-tag Protein Binding Activity

When Recombinant Mouse CTRP9/C1qTNF9 (Catalog # 2132-TN) is coated at 0.5 μg/mL, 100 μL/well, Biotinylated Recombinant Human CL-K1/COLEC11 binds with an ED50 of 15-90 ng/mL.
Recombinant Mouse CTRP9/C1qTNF9 His-tag Protein SDS-PAGE

Recombinant Mouse CTRP9/C1qTNF9 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Mouse CTRP9/C1qTNF9 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 39-43 kDa and 39-43 & 80-90 kDa, respectively.

Formulation, Preparation and Storage

2132-TN
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CTRP9/C1qTNF9

C1qTNF9, also known as CTRP9, is an approximately 40 kDa member of the C1q and TNF-related protein family (1). Like all members of this protein family, C1qTNF9 consists of a short variable region, a collagenous domain that can be hydroxylated, and a C1q-like globular domain (1). Mouse C1qTNF9 shares 86% and 98% amino acid sequence identity with the human and rat orthologs, respectively. Both the mouse and human C1qTNF9 proteins are expressed mostly in adipose tissue, but the mouse protein has also been detected in the heart, lung, muscle, kidney, testis, lymph node, smooth muscle, prostate, thymus, and uterus (1, 2). C1qTNF9 has been shown to be secreted as trimers and higher order multimers and also to form hetero-oligomers with Adiponectin (1, 2). Mouse C1qTNF9 can stimulate the phosphorylation of AMPK, Akt, and eNOS (1, 3, 4). Also in mice, C1qTNF9 may have an important role in cardiac and metabolic health. Its expression has a cardioprotective effect following acute myocardial infarction that may be dependent on AMPK activation (4-6). Additionally, transgenic mice overexpressing C1qTNF9 are resistant to high fat diet‑induced obesity (7). This metabolic role may be conserved, since C1qTNF9 serum levels have been shown to inversely correlate with metabolic syndrome in humans (8).

References

  1. Wong, G.W. et al. (2009) FASEB J. 23:241.
  2. Peterson, J.M. et al. (2009) Biochem. Biophys. Res. Commun. 388:360.
  3. Zheng, Q. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:2616.
  4. Kambara, T. et al. (2012) J. Biol. Chem. 287:18965.
  5. Su, H. et al. (2013) Basic Res. Cardiol. 108:315.
  6. Sun, Y. et al. (2013) Circulation 128:S113.
  7. Peterson, J.M. et al. (2013) Am. J. Physiol. Regul. Integr. Comp. Physiol. 305: R522.
  8. Hwang, Y.C. et al. (2014) Int. J. Obes. (Lond) 38:1207

Long Name

C1q and Tumor Necrosis Factor Related Protein 9

Alternate Names

AQL1, C1qTNF9

Entrez Gene IDs

338872 (Human); 239126 (Mouse)

Gene Symbol

C1QTNF9

UniProt

Q4ZJN1

Additional CTRP9/C1qTNF9 Products

Product Documents for Recombinant Mouse CTRP9/C1qTNF9 His-tag Protein, CF

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Product Specific Notices for Recombinant Mouse CTRP9/C1qTNF9 His-tag Protein, CF

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