Recombinant Mouse DDR1 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6416-DR

R&D Systems, part of Bio-Techne
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6416-DR-050
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Key Product Details

Source

NS0

Accession #

Q03146

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all DDR1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse DDR1 protein
Mouse DDR1
(Met1 - Thr414)
Accession # Q03146		 IEGRMDP Mouse IgG2A
(Glu98 - Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp22

Predicted Molecular Mass

71.1 kDa (monomer)

SDS-PAGE

85-95 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized Collagen I at 10 µg/mL (100 µL/well) can bind Recombinant Mouse DDR1 Fc Chimera with an apparent KD < 20 nM.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

6416-DR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 300 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DDR1

DDR1, also known as CAK, NEP, CD167a, and EDDR1, is a 120 - 140 kDa type I transmembrane glycoprotein belonging to the discoidin-like domain‑containing subfamily of receptor tyrosine kinases (1, 2). Mature mouse DDR1 consists of a 395 aa extracellular domain (ECD) that includes the discoidin-like domain, a 27 aa transmembrane segment, and a 470 aa cytoplasmic domain with the tyrosine kinase domain (3). Within the ECD, mouse DDR1 shares 93% aa sequence identity with human and rat DDR1, respectively. Alternate splicing of mouse DDR1 generates an additional isoform that lacks a portion of the cytoplasmic domain encompassing the Shc-interacting NPxY motif (4). DDR1 is expressed on epithelial tissues, activated monocytes and neutrophils, smooth muscle cells, and in several cancers (1, 2). It mediates cellular adhesion and migration through interaction of the discoidin-like domain with the triple helical structure of collagens I ‑ V (5, 6). Collagen binding induces prolonged tyrosine autophosphorylation of DDR1, including within the NPxY motif (5, 6). Collagen binding can also induce the metalloproteinase-dependent cleavage of DDR1, thereby liberating a tyrosine phosphorylated 60 kDa C-terminal fragment and a 60 kDa ECD fragment (7, 8). DDR1 is expressed as a dimer on the cell surface independently of ligand binding (9). Oligomerization enhances collagen binding and also modulates collagen fibrillogenesis (10, 11). Collagen-induced activation of DDR1 is inhibited by the association of DDR1 with E-Cadherin at epithelial cell junctions (12). Expression of DDR1 on arterial smooth muscle cells limits smooth muscle cell migration and the development of proteoglycan plaques during atherogenesis (13). The overexpression of particular DDR1 isoforms in glioblastoma promotes MMP-2 activation and increased tumor cell invasiveness (14).

References

  1. Vogel, W.F. et al. (2006) Cell. Signal. 18:1108.
  2. Yoshimura, T. et al. (2005) Immunol. Res. 31:219.
  3. Zerlin, M. et al. (1993) Oncogene 8:2731.
  4. Perez, J.L. et al. (1996) Oncogene 12:1469.
  5. Shrivastava, A. et al. (1997) Mol. Cell 1:25.
  6. Vogel, W. et al. (1997) Mol. Cell 1:13.
  7. Slack, B.E. et al. (2006) J. Cell Biochem. 98:672.
  8. Vogel, W.F. et al. (2001) FEBS Lett. 514:175.
  9. Noordeen, N.A. et al. (2006) J. Biol. Chem. 281:22744.
  10. Leitinger, B. (2003) J. Biol. Chem. 278:16761.
  11. Agarwal, G. et al. (2007) J. Mol. Biol. 367:443.
  12. Wang, C.Z et al. (2009) Am. J. Physiol. Cell Physiol. 297:C419.
  13. Franco, C. et al. (2010) Circ. Res. 106:1775.
  14. Ram, R. et al. (2006) J. Neurooncol. 76:239.

Long Name

Discoidin Domain Receptor 1

Alternate Names

CAK, CD167a, RTK6, TrkE

Entrez Gene IDs

780 (Human)

Gene Symbol

DDR1

UniProt

Q03146

Additional DDR1 Products

Product Documents for Recombinant Mouse DDR1 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse DDR1 Fc Chimera Protein, CF

For research use only

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