Recombinant Mouse DR6/TNFRSF21 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6985-DR

R&D Systems, part of Bio-Techne
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6985-DR-050
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Key Product Details

Source

NS0

Accession #

NP_848704

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all DR6/TNFRSF21 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse DR6/TNFRSF21 protein
Mouse DR6
(Met1 - His349)
Accession # NP_848704		 IEGRMDP Mouse IgG2A
(Glu98 - Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln42 predicted, N-sequencing might be blocked

Predicted Molecular Mass

60.7 kDa (monomer)

SDS-PAGE

80-95 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.

When recombinant human APP770 is coated at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse DR6/TNFRSF21 Fc Chimera that produces 50% of the optimal binding response is found to be approximately 80-400 ng/mL.

Formulation, Preparation and Storage

6985-DR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DR6/TNFRSF21

Death Receptor 6 (DR6), also known as TNFRSF21 and CD358, is a type I transmembrane protein in the TNF receptor superfamily (1). Mouse DR6 consists of a 308 amino acid (aa) extracellular domain (ECD) with four cysteine-rich motifs, a 21 aa transmembrane segment, and a 285 aa palmityolated cytoplasmic region that contains one death domain (2, 3). Within the ECD, mouse and human DR6 share 82% aa sequence identity. DR6 is expressed as an approximately 110 kDa molecule that carries extensive N-linked and O-linked glycosylation in its extracellular region (3, 4). Among hematopoietic cells, DR6 is expressed on monocytes, resting CD4+ T cells, and pro-, pre-, and naïve B cells (5 - 7). DR6 knockout mice exhibit a Th2-biased immune response characterized by exaggerated Th2 and B cell responsiveness in combination with reduced Th1 cell responsiveness and inflammatory leukocyte infiltration (2, 6 - 8). DR6 knockout mice are resistant to induced airway inflammation and experimental autoimmune encephalitis but more susceptible to severe graft versus host disease (8 - 10). DR6 is also expressed on developing neurons where it can bind a shed 35 kDa N-terminal fragment of APP or a fragment of APLP2 (11, 12). This APP fragment is generated following deprivation of neurotrophic factors, and its binding to DR6 triggers DR6-mediated axonal pruning (11). DR6 is constitutively expressed on some prostate cancer cells and can be induced by TNF-alpha on others (3, 4).

References

  1. Benschop, R. et al. (2009) Adv. Exp. Med. Biol. 647:186.
  2. Zhao, H. et al. (2001) J. Exp. Med. 194:1441.
  3. Klima, M. et al. (2009) Biochim. Biophys. Acta 1793:1579.
  4. Kasof, G.M. et al. (2001) Oncogene 20:7965.
  5. Matesanz-Isabel, J. et al. (2011) Immunol. Lett. 134:104.
  6. Schmidt, C.S. et al. (2003) J. Exp. Med. 197:51.
  7. Liu, J. et al. (2001) Immunity 15:23.
  8. Venkataraman, C. et al. (2006) Immunol. Lett. 106:42.
  9. Schmidt, C.S. et al. (2005) J. Immunol. 175:2286.
  10. Liu, J. et al. (2002) J. Immunol. 169:3993.
  11. Nikolaev, A. et al. (2009) Nature 457:981.
  12. Kuester, M. et al. (2011) J. Mol. Biol. 409:189.

Long Name

Death Receptor 6

Alternate Names

BM-018, CD358, TNFRSF21

Entrez Gene IDs

27242 (Human); 94185 (Mouse); 316256 (Rat)

Gene Symbol

TNFRSF21

UniProt

NP_848704

Additional DR6/TNFRSF21 Products

Product Documents for Recombinant Mouse DR6/TNFRSF21 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse DR6/TNFRSF21 Fc Chimera Protein, CF

For research use only

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