Recombinant Mouse EGF-L6 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 4329-EG

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
4329-EG-025
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

NS0

Accession #

Q9JJZ5

Conjugate

Unconjugated

Applications

Bioactivity

View all EGF-L6 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse EGF-L6 protein
Thr287-Gly550 & Lys291-Gly550, both with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr287 & Lys291

Predicted Molecular Mass

30 kDa

SDS-PAGE

37-40 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. Osada, A. et al. (2005) Exp. Cell Res. 303:148.
Recombinant Mouse EGF-L6 immobilized at 2 µg/mL, 100 µL/well, will mediate >60% NIH/3T3 cell adhesion (100 µL/well at 5 x 105 cells/mL).

Formulation, Preparation and Storage

4329-EG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EGF-L6

Epidermal Growth Factor-like Domain, Multiple 6 (EGF-L6), also known as MAM- and EGF-containing (MAEG) is a member of the EGF repeat superfamily of proteins. This secreted protein contains five EGF-like repeats, an RGD motif that binds to Integrin alpha8 beta1, and a C-terminal MAM domain (1, 2). Within shared regions, mouse EGF-L6 shares 75% and 91% amino acid sequence identity with the human and rat orthologs, respectively. EGF-L6 is expressed in a multitude of fetal tissues during early development such as lung, heart, liver, spleen, cochlea, and placenta (3, 4). It is also expressed in the dermatome and dermatome-derived tissues including trunk dermis, hair follicles, and cranio-facial mesenchyme (5). EGF-L6 is expressed by osteoblastic-like cells and has been shown to act in a paracrine manner to promote migration of vascular endothelial cells, thus regulating angiogenesis in the local environment of the bone (4, 6). Additionally, EGF-L6 is expressed by epidermal stem cells in the hair follicle bulge and is believed to play a role in regulating the dermal vasculature (7). EGF-L6 is also produced by mature adipocytes and is thought to be involved in the process of adipose tissue expansion and the development of obesity (8). EGF-L6 expression is increased in fibroblastic meningioma and ovarian cancer and has been proposed as a biomarker for the latter (9, 10).

References

  1. Buchner, G. et al. (2000) Genomics 65:16.
  2. Osada, A. et al. (2005) Exp. Cell Res. 303:148.
  3. Yeung, G. et al. (1999) Genomics 62:304.
  4. Chim, S.M. et al. (2011) J. Biol. Chem. 286:22035.
  5. Buchner, G. et al. (2000) Mech. Dev. 98:179.
  6. Chim, S.M. et al. (2013) Cytokine Growth Factor Rev. 24:297.
  7. Xiao, Y. et al. (2013) J. Invest. Dermatol. 133:2324.
  8. Oberauer, R. et al. (2010) Mol. Cell. Biochem. 343:257.
  9. Buckanovich, R.J. et al. (2007) J. Clin. Oncol. 25:852.
  10. Wang, X. et al. (2012) PLoS ONE 7:e52707.

Long Name

EGF-like Domain, Multiple 6

Alternate Names

EGFL6, MAEG

Entrez Gene IDs

25975 (Human); 54156 (Mouse); 317470 (Rat)

Gene Symbol

EGFL6

UniProt

Q9JJZ5

Additional EGF-L6 Products

Product Documents for Recombinant Mouse EGF-L6 Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Mouse EGF-L6 Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.