Recombinant Mouse Endoglycan/PODXL2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8365-EG

R&D Systems, part of Bio-Techne
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8365-EG-050
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Key Product Details

Source

NS0

Accession #

XP_006506309

Conjugate

Unconjugated

Applications

Bioactivity

View all Endoglycan/PODXL2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Endoglycan/PODXL2 protein
Gly30-Gly498, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly30

Predicted Molecular Mass

51 kDa

SDS-PAGE

90-124 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to inhibit the adhesion of CHO Chinese hamster ovary cells transfected with P-Selectin.
The ED50 for this effect is 0.1-0.6 μg/mL.

Formulation, Preparation and Storage

8365-EG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endoglycan/PODXL2

Endoglycan, also called Podocalyxin-like 2 protein (PODXL2), is an extensively O-glycosylated type I transmembrane protein in the CD34 family of sialomucins (1, 2). Mature mouse Endoglycan consists of an extracellular domain (ECD) that contains an N-terminal acidic domain characterized by several polyglutamate tracts, a mucin domain, and a membrane-proximal globular domain. Within the ECD, mouse Endoglycan shares 96% and 75% amino acid identity with rat and human Endoglycan, respectively. Following the extracellular domain is a single transmembrane spanning helix and a charged cytoplasmic tail that contains a C-terminal PDZ-domain binding motif (1, 3). The cytoplasmic domain of Endoglycan has been shown to bind to Na+/H+ exchanger regulatory factor-1 (NHERF-1), which acts as a scaffolding protein that links membrane proteins to the actin cytoskeleton (4). Endoglycan exists as a disulfide-linked homodimer that is expressed in vascular endothelium, smooth muscle, hematopoietic precursor populations and leukocyte subpopulations (1, 5). Endoglycan functions through its interactions with selectins and is involved in leukocyte-endothelial cell adhesion although, depending on cellular context and post-translational modifications, Endoglycan may also demonstrate anti-adhesive properties (2, 3, 5, 6).

References

  1. Sassetti, C. et al. (2000) J. Biol. Chem. 275:9001.
  2. Fieger, C.B. et al. (2003) J. Biol. Chem. 278:27390.
  3. Nielsen, J.S. and K.M. McNagny (2008) J. Cell Sci. 121:3683.
  4. Tan, P.C. et al. (2006) Stem Cells 24:1150.
  5. Kerr, S.C. et al. (2008) J. Immunol. 181:1480.
  6. Sarangapani, K.K. et al. (2004) J. Biol. Chem. 279:2291.

Alternate Names

PODLX2, PODXL2

Entrez Gene IDs

50512 (Human); 319655 (Mouse)

Gene Symbol

PODXL2

UniProt

XP_006506309

Additional Endoglycan/PODXL2 Products

Product Documents for Recombinant Mouse Endoglycan/PODXL2 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse Endoglycan/PODXL2 Protein, CF

For research use only

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