Recombinant Mouse Endorepellin His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9987-ER

R&D Systems, part of Bio-Techne
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9987-ER-050
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Key Product Details

Source

CHO

Accession #

NP_032331.2

Conjugate

Unconjugated

Applications

Bioactivity

View all Endorepellin/Perlecan Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Endorepellin/Perlecan protein
Glu3683-Ser4383, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu3683

Predicted Molecular Mass

75.8 kDa

SDS-PAGE

85-95 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of SVEC4-10 mouse vascular endothelial cells.
The ED50 for this effect is 0.75-4.5 μg/mL.

Scientific Data Images for Recombinant Mouse Endorepellin His-tag Protein, CF

Recombinant Mouse Endorepellin His-tag Protein Bioactivity

Recombinant Mouse Endorepellin His-tag Protein Bioactivity

Immobilized Recombinant Mouse Endorepellin supports the adhesion of SVEC4‑10 mouse vascular endothelial cells. The ED50 for this effect is 0.75-4.5 μg/mL.
Recombinant Mouse Endorepellin His-tag Protein SDS-PAGE

Recombinant Mouse Endorepellin His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Mouse Endorepellin/Perlecan was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 85-95 kDa.

Formulation, Preparation and Storage

9987-ER
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 300 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: Endorepellin/Perlecan

Endorepellin is an 80 kDa glycoprotein derived from the C-terminal end of the heparan sulfate proteoglycan Perlecan. Human Perlecan is an approximately 850 kDa basement membrane heparan sulfate proteoglycan containing multiple LDLR, EGF-like, Laminin-like, and immunoglobulin-like domains. Mouse Perlecan lacks several of the EGF, Laminin-like and Ig-like domains found in the human protein (1-3). Mouse Endorepellin, also termed Domain V (DV) of Perlecan, consists of three Laminin G domains separated by a single EGF-like domain. Mouse Endorepellin shares 89% amino acid sequence identity with human Endorepellin. Endorepellin has been shown to broadly inhibit angiogenesis, including endothelial cell migration, collagen-induced capillary morphogenesis, and blood vessel growth (4). Endostatin, an inhibitor of Endorepellin, has been shown to bind Endorepellin directly and block its anti-angiogenic activity (4). Endorepellin can be further processed into a 26 kDa fragment, termed LG3, containing the third Laminin G-like domain of Endorepellin. LG3 possesses anti-angiogenic activity of its own and its release is mediated by either Cathepsin L or BMP-1/Tolloid family cleavage (4-7). Endorepellin binds to Integrin alpha 2/ beta 1, preventing the integrin-dependent adhesion of vascular endothelial cells (EC) to fibronectin and collagen I (7, 8). Additionally, Endorepellin binds to VEGF R1 and VEGF R2 on EC (10). Its binding to VEGF R2 and Integrin alpha 2/beta 1 on EC induces the association and down‑regulation of both proteins, resulting in a reduction in EC migration and overall antiangiogenic activity (4, 7-10). Endorepellin has been shown to disrupt tumor vasculature and inhibit tumor growth, invasion and angiogenesis (8, 11), and it has recently been proposed as a potential stroke therapy by helping with brain repair following injury (12).

References

  1. Whitelock, J.M. et al. (2008) Biochemistry 47:11174.
  2. Murdoch, A.D. et al. (1992) J. Biol. Chem. 267:8544.
  3. Noonan, D.M. et al. (1991) J. Biol. Chem. 266:22939.
  4. Mongiat, M. et al. (2003) J. Biol. Chem. 278:4238.
  5. Cailhier, J.-F. et al. (2008) J. Biol. Chem. 283:27220.
  6. Gonzalez, E.M. et al. (2005) J. Biol. Chem. 280:7080.
  7. Bix, G. et al. (2004) J. Cell Biol. 166:97.
  8. Woodall, B.P. et al. (2008) J. Biol. Chem. 283:2335.
  9. Goyal, A. et al. (2011) J. Biol. Chem. 286:25947.
  10. Nystrom, A. et al. (2009) Blood 114:4897.
  11. Bix, G. et al. (2006) J. Natl. Cancer Inst. 98:1634.
  12. Bix G. (2013) ACS Chem Neurosci. 4:370.

Long Name

Heparan Sulfate Proteoglycan

Alternate Names

HSPG2, Perlecan

Entrez Gene IDs

3339 (Human); 15530 (Mouse)

Gene Symbol

HSPG2

UniProt

NP_032331.2

Additional Endorepellin/Perlecan Products

Product Documents for Recombinant Mouse Endorepellin His-tag Protein, CF

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Product Specific Notices for Recombinant Mouse Endorepellin His-tag Protein, CF

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