Recombinant Mouse Ephrin-A2 Fc Chimera Biotinylated Protein

R&D Systems, part of Bio-Techne | Catalog # BT603

R&D Systems, part of Bio-Techne
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BT603
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Key Product Details

Source

NS0

Accession #

P52801

Structure / Form

Disulfide-linked homodimer

Conjugate

Biotin

Applications

Binding Activity

View all Ephrin-A2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Ephrin-A2 protein
Human Ephrin-A2
(Glu23-Asn184)
Accession # P52801		 IEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

N-terminal Sequence Analysis

Glu23

Predicted Molecular Mass

46 kDa (monomer)

SDS-PAGE

57 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized recombinant rat EphA5 Fc Chimera at 2 µg/mL can bind biotinylated rmEphrin-A2 with a linear range of 0.16-10 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

BT603
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution

Reconstitute at 100 μg/mL with sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ephrin-A2

Ephrin-A2, also known as ELF-1, HEK7-L, LERK-6, and EPLG6, is an approximately 20 kDa member of the Ephrin-A family of GPI-anchored ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. Ephrin-A ligands are structurally related to the extracellular domains of the transmembrane Ephrin-B ligands. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin-A2 preferentially interacts with receptors in the EphA family (1, 2). Mouse Ephrin-A2 is synthesized with a 20 amino acid (aa) signal peptide, a 164 aa mature chain, and a 25 aa C-terminal propeptide which is removed prior to GPI linkage of Ephrin-A2 to the membrane (3, 4). It shares 93% and 100% aa sequence identity with human and rat Ephrin-A2, respectively. Ephrin-A2 is expressed in discrete regions of the developing nervous system and limb buds (4 - 7). Its distribution complements the pattern of Eph receptor expression, and this plays an important role in tissue morphogenesis (7‑9). Ephrin-A2 exerts an axon repulsive signal which is important for the accurate pathfinding of retinal ganglion cell axons to the tectum and hippocampal axons to the lateral septum (8, 10). Its up‑regulation in astrocytes at sites of optic nerve damage may prevent re-innervation by retinal ganglion cells (11). Ephrin-A2 is also expressed on neural progenitor cells in the subventricular zone (SVZ). It interacts with EphA7, triggering reverse signaling through Ephrin-A2 and inhibition of progenitor cell proliferation (9). In the developing limbs, Ephrin-A2 regulates cartilage morphogenesis and the projection of motoneuron axons (6, 7, 12).

References

  1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
  2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
  3. Shao, H. et al. (1995) J. Biol. Chem. 270:3467.
  4. Cheng, H.-J. et al. (1994) Cell 79:157.
  5. Kenmuir, C.L. et al. (2012) Anat. Rec. (Hoboken) 295:105.
  6. Ohta, K. et al. (1997) Mech. Dev. 64:127.
  7. Wada, N. et al. (2003) Dev. Biol. 264:550.
  8. Gao, P.-P. et al. (1996) Proc. Natl. Acad. Sci. USA 93:11161.
  9. Holmberg, J. et al. (2005) Genes Dev. 19:462.
  10. Nakamoto, M. et al. (1996) Cell 86:755.
  11. Symonds, A.C.E. et al. (2007) Eur. J. Neurosci. 25:744.
  12. Eberhart, J. et al. (2000) Dev. Neurosci. 22:237.

Alternate Names

Cek7-L, EFNA2, ELF-1, EphrinA2, LERK6

Entrez Gene IDs

1943 (Human); 13637 (Mouse)

Gene Symbol

EFNA2

UniProt

P52801

Additional Ephrin-A2 Products

Product Documents for Recombinant Mouse Ephrin-A2 Fc Chimera Biotinylated Protein

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Product Specific Notices for Recombinant Mouse Ephrin-A2 Fc Chimera Biotinylated Protein

For research use only

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