Recombinant Mouse Ephrin-A5 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7396-EA

R&D Systems, part of Bio-Techne
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7396-EA-050
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Key Product Details

Source

NS0

Accession #

NP_997537

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Ephrin-A5 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Ephrin-A5 protein
Mouse Ephrin-A5
(Met1-Asn203)
Accession # NP_997537		 IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln21 predicted, sequencing might be blocked

Predicted Molecular Mass

48.4 kDa (monomer)

SDS-PAGE

57-60 kDa, reducing conditions

Activity

Measured by its ability to inhibit proliferation of PC-3 human prostate cancer cells.
The ED50 for this effect is 2.5-10 ng/mL.

Formulation, Preparation and Storage

7396-EA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ephrin-A5

Ephrin‑A5, also known as AL‑1, RAGS, and LERK‑7, is an approximately 25 kDa member of the Ephrin‑A family of GPI‑anchored ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. Ephrin‑A ligands are structurally related to the extracellular domains of the transmembrane Ephrin‑B ligands. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1, 2). Ephrin‑A5 preferentially interacts with receptors in the EphA family but also with EphB2 (3). Mature mouse Ephrin‑A5 shares 98.9% and 99.5% aa sequence identity with human and rat Ephrin‑A5 (4). Alternate splicing of mouse Ephrin‑A5 generates a short isoform that lacks 27 amino acids in the juxtamembrane region. The short isoform retains the ability to bind EphA3 and inhibit neurite extension (5). Ephrin‑A5 is expressed in multiple tissues during development, particularly in the brain (6, 7). It can exert repulsive or attractive effects on migrating neurons in the developing brain and motor column of the spinal cord (7‑11). Ephrin‑A5 repels migrating axons by inducing growth cone collapse and neurite retraction and by inhibiting the neurotrophic effects of NGF and BDNF (3, 12, 13). It interacts in cis with EphA3 on retinal axon growth cones which reduces axonal sensitivity to Ephrin‑A5 in trans (14). In the adult, Ephrin‑A5 is expressed on hippocampal neurons and astrocytes and induces the development of hippocampal synapses (10, 15, 16). It supports the proliferation of neural progenitors and the survival of newly differentiated neurons (15). Ephrin‑A5 functions as a tumor suppressor and its normal function in inhibiting EGFR signaling is compromised by its down‑regulation in glioma (17). Ephrin A5 is also down‑regulated in prostate cancer (18). Ephrin‑A5 is expressed by muscle precursor cells and interacts with EphA4 to restrict their migration to the correct locations during forelimb morphogenesis (19).

References

  1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
  2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
  3. Himanen, J.-P. et al. (2004) Nat. Neurosci. 7:501.
  4. Flenniken, A.M. et al. (1996) Dev. Biol. 179:382.
  5. Lai, K.-O. et al. (1999) FEBS Lett. 458:265.
  6. Deschamps, C. et al. (2010) BMC Neurosci. 11:105.
  7. Cooper, M.A. et al. (2009) Dev. Neurobiol. 69:36.
  8. Frisen, J. et al. (1998) Neuron 20:235.
  9. Zimmer, G. et al. (2008) Eur. J. Neurosci. 28:62.
  10. Otal, R. et al. (2006) Neuroscience 141:109.
  11. Eberhart, J. et al. (2004) J. Neurosci. 24:1070.
  12. Munoz, L.M. et al. (2005) Dev. Biol. 283:397.
  13. Meier, C. et al. (2011) PLoS ONE 6:e26089.
  14. Carvalho, R.F. et al. (2006) Nat. Neurosci. 9:322.
  15. Hara, Y. et al. (2010) Stem Cells 28:974.
  16. Akaneya, Y. et al. (2010) PLoS ONE 5:e12486.
  17. Li, J.-J. et al. (2009) Oncogene 28:1759.
  18. Gustavsson, H. et al. (2008) Prostate 68:161.
  19. Swartz, M.E. et al. (2001) Development 128:4669.

Alternate Names

AL-1, EFL-5, EFNA5, EphrinA5, LERK-7, RAGS

Entrez Gene IDs

1946 (Human); 13640 (Mouse)

Gene Symbol

EFNA5

UniProt

NP_997537

Additional Ephrin-A5 Products

Product Documents for Recombinant Mouse Ephrin-A5 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse Ephrin-A5 Fc Chimera Protein, CF

For research use only

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