Recombinant Mouse Fc alpha/mu R His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10069-FC

R&D Systems, part of Bio-Techne
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10069-FC-050
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Key Product Details

Source

NS0

Accession #

Q2TB54-1

Conjugate

Unconjugated

Applications

Bioactivity

View all Fc alpha/mu R Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Fc alpha/mu R protein
Trp47-Arg455, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Trp47

Predicted Molecular Mass

44 kDa

SDS-PAGE

97-112 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Fc alpha/μ R is immobilized at 2 μg/mL, 100 μL/well, the concentration of Mouse IgM that produces 50% of the optimal binding response is 0.04-0.24 μg/mL.

Scientific Data Images for Recombinant Mouse Fc alpha/mu R His-tag Protein, CF

Recombinant Mouse Fc alpha/mu R His-tag Protein Binding Activity

Recombinant Mouse Fc alpha/mu R His-tag Protein Binding Activity

When Recombinant Mouse Fc alpha/mu R (Catalog # 10069-FC) is coated at 2ug/mL, 100 uL/well, Mouse IgM binds with an ED50 of 0.04-0.24 μg/mL.

Formulation, Preparation and Storage

10069-FC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: Fc alpha/mu R

Fc alpha/mu Receptor (FCAMR), designated CD351, is an approximately 60-kDa transmembrane protein that serves as a receptor for IgA and IgM immunoglobulins (1). Mature mouse FCAMR consists of a 420 amino acid (aa) extracellular domain with one Ig-like V-type domain, a 21 aa transmembrane segment, and a 59 aa cytoplasmic domain. Within the ECD, mouse FCAMR shares 51% aa and 80% aa sequence identity with human and rat FCAMR, respectively. FCAMR is expressed on B cells, macrophages, and kidney mesangial cells (2-4). It binds to both IgA and IgM in immune complexes but not to monomeric immunoglobulin (2, 5). FCAMR participates in pathogen clearance as well as foam cell formation by mediating the internalization of IgM-opsonized microbes and oxidized LDL-containing particles (2, 6).

References

  1. Wang, H. et al. (2016) Front. Immunol. 7:99.
  2. Shibuya, A. et al. (2000) Nat. Immunol. 1:441.
  3. Matesanz-Isabel, J. et al. (2011) Immunol. Lett. 134:104.
  4. McDonald, K.J. et al. (2002) Biochem. Biophys. Res. Commun. 290:438.
  5. Ghumra, A. et al. (2009) Eur. J. Immunol. 39:1147.
  6. Feng, X. et al. (2010) Atherosclerosis 208:396.

Long Name

Fc alpha/mu Receptor

Alternate Names

CD351, FCA/MR, FCAMR, FKSG87, uR

Entrez Gene IDs

83953 (Human); 64435 (Mouse); 681070 (Rat)

Gene Symbol

FCAMR

UniProt

Q2TB54-1

Additional Fc alpha/mu R Products

Product Documents for Recombinant Mouse Fc alpha/mu R His-tag Protein, CF

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Product Specific Notices for Recombinant Mouse Fc alpha/mu R His-tag Protein, CF

For research use only

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