Recombinant Mouse FCRL1/FcRH1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7286-FC

R&D Systems, part of Bio-Techne
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7286-FC-050
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Key Product Details

Source

NS0

Accession #

Q8R4Y0

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all FCRL1/FcRH1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse FCRL1/FcRH1 protein
Met1-Ser219, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala17

Predicted Molecular Mass

22.5 kDa (monomer)

SDS-PAGE

40-60 kDa, reducing conditions

Activity

Measured by its ability to bind mouse IgG with an estimated KD <750 nM.

Formulation, Preparation and Storage

7286-FC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FCRL1/FcRH1

Fc Receptor‑Like 1 (FCRL1), also known as FcRH1, IRTA5, and IFGP1, is an approximately 50 kDa protein with sequence homology to classical Fc receptors. The type 1 transmembrane FCRL proteins contain from three to nine immunoglobulin‑like domains. They are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1, 2). According to R&D Systems testing, FCRL1 binds to purified mouse IgG. Mature mouse FCRL1 consists of a 203 amino acid (aa) extracellular domain (ECD) with two Ig‑like domains, a 21 aa transmembrane segment, and a 103 aa cytoplasmic domain with two immunotyrosine activation motifs (ITAMs) (3, 4). Alternative splicing may generate additional isoforms that lack the transmembrane segment, have a substituted cytoplasmic domain, or essentially consist of the first two Ig‑like domains (4). The ECD of mouse FCRL1 shares 81% aa sequence identity with rat FCRL1 and 62% with comparable regions of human FCRL1 which contains three Ig‑like domains. FCRL1 is expressed on pre‑B cells and naive B cells (5‑7). It is down‑regulated upon B cell activation but up‑regulated on memory B cells (5, 6, 8). FCRL1 is expressed on many B cell lymphoma and leukemia tumor cells with the exception of B cell acute lymphoblastic leukemia (5, 9‑11). Antibody crosslinking of FCRL1 triggers its tyrosine phosphorylation and augments B cell proliferation induced by the B cell receptor (6).

References

  1. Ehrhardt, G.R. and M.D. Cooper (2011) Curr. Top. Microbiol. Immunol. 350:89.
  2. Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.
  3. Guselnikov, S.V. et al. (2002) Immunogenetics 54:87.
  4. Davis, R.S. et al. (2004) Int. Immunol. 16:1343.
  5. Matesanz-Isabel, J. et al. (2011) Immunol. Lett. 134:104.
  6. Leu, C.-M. et al. (2005) Blood 105:1121.
  7. Li, F.J. et al. (2008) Blood 112:179.
  8. Polson, A.G. et al. (2006) Int. Immunol. 18:1363.
  9. Du, X. et al. (2008) Blood 111:338.
  10. Kazemi, T. et al. (2008) Int. J. Cancer 123:2113.
  11. Kazemi, T. et al. (2009) Cancer Immunol. Immunother. 58:989.

Long Name

Fc Receptor-like 1

Alternate Names

BXMH1, CD307a, FcRH1, IFGP1, IRTA5

Entrez Gene IDs

115350 (Human); 229499 (Mouse); 680665 (Rat)

Gene Symbol

FCRL1

UniProt

Q8R4Y0

Additional FCRL1/FcRH1 Products

Product Documents for Recombinant Mouse FCRL1/FcRH1 Protein, CF

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Product Specific Notices for Recombinant Mouse FCRL1/FcRH1 Protein, CF

For research use only

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