Recombinant Mouse FCRLB/FCRY Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 4868-FC

R&D Systems, part of Bio-Techne
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4868-FC-050
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Key Product Details

Source

NS0

Accession #

Q5DRQ8

Conjugate

Unconjugated

Applications

Bioactivity

View all FCRLB/FCRY Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse FCRLB/FCRY protein
Ala18-Ser427, with a C-terminal 6-His tag

Purity

>80%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala18

Predicted Molecular Mass

46.5 kDa (monomer)

SDS-PAGE

60-70 kDa, reducing conditions

Activity

Measured by its ability to bind mouse IgG with an estimated KD < 300 nM.

Formulation, Preparation and Storage

4868-FC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FCRLB/FCRY

FCRLB, also known as FCRL2, FCRY, and FREB2, is a 60 kDa protein with sequence homology to classical Fc receptors. There are at least six type 1 transmembrane FCRL proteins and two that lack transmembrane segments. Each family member contains between three and nine immunoglobulin-like domains. FCRL proteins are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1, 2). According to R&D Systems testing, FCRLB binds to purified mouse IgG. Mouse FCRLB shares 82% and 94% aa sequence identity with human and rat FCRLB, respectively. It contains a putative signal peptide, three immunoglobulin-like domains, and a mucin-like stalk that is rich in Pro, Ser, and Thr residues (1 - 3). When expressed in transfectants, FCRLB is not secreted but shows a diffuse intracellular localization (4, 5). FCRLB is expressed at a low level in placenta and in B lineage cells of the germinal center (4, 5). It is up‑regulated in B cells by BAFF and LPS (3). Among non‑hematopoietic cells, FCRLB is expressed in fibroblasts, melanocytes, and melanoma (3, 4, 6). It is preferentially expressed in non‑proliferating cells and at the onset of apoptosis (3, 5).

References

  1. Davis, R.S. (2007) Annu. Rev. Immunol. 25:525.
  2. Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.
  3. Masuda, K. et al. (2005) Gene 363:32.
  4. Chikaev, N.A. et al. (2005) Genomics 85:264.
  5. Wilson, T.J. and M. Colonna (2005) Genes Immun. 6:341.
  6. Inozume, T. et al. (2005) Int. J. Cancer 114:283.

Long Name

Fc Receptor-like B

Alternate Names

FcRL2, FCRLM2, FCRLY, FCRY, FREB-2

Entrez Gene IDs

127943 (Human); 435653 (Mouse); 498275 (Rat)

Gene Symbol

FCRLB

UniProt

Q5DRQ8

Additional FCRLB/FCRY Products

Product Documents for Recombinant Mouse FCRLB/FCRY Protein, CF

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Product Specific Notices for Recombinant Mouse FCRLB/FCRY Protein, CF

For research use only

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