Recombinant Mouse FGF-6 Protein

R&D Systems, part of Bio-Techne | Catalog # 5750-F6

R&D Systems, part of Bio-Techne
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Carrier Free
5750-F6-025/CF
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With Carrier
5750-F6-025
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Key Product Details

Source

E. coli

Accession #

NP_034334

Conjugate

Unconjugated

Applications

Bioactivity

View all FGF-6 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived mouse FGF-6 protein
Pro39-Ile208

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Pro39

Predicted Molecular Mass

18.8 kDa

SDS-PAGE

19 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749.
The ED50 for this effect is 0.2-1.0 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 5750-F6
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Ammonium Sulfate, EDTA, DTT with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 5750-F6/CF
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Ammonium Sulfate, EDTA, DTT.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-6

Fibroblast Growth Factor-6 (FGF-6), also known as HST-2, is a 25 - 28 kDa member of the FGF family of heparin binding polypeptides which are potent regulators of cell proliferation, differentiation, and function. FGF proteins contain a 120 amino acid (aa) core FGF domain that exhibits a beta-trefoil structure (1, 2). Mature mouse FGF-6 is a 171 aa protein that shares 94% and 99% aa sequence identity with human and rat FGF-6, respectively (3). It binds and signals primarily through FGF R1c, 2c, and 4 (4). FGF-6 functions as a mitogen for fibroblasts, vascular endothelial cells, and prostate carcinoma cells, and N-linked glycosylation is required for the full mitogenic effect (5 - 7). FGF-6 expression is restricted to skeletal muscle during development, although it can be upregulated in prostate cancer and Kaposi sarcoma (7 - 9). In the adult, FGF-6 is upregulated in injured skeletal muscle and is required for muscle regeneration (10). FGF-6 inhibits the terminal differentiation of myoblasts and also cooperates with TGF-beta 2 to promote chondrogenesis in embryonic somites (8, 11).

References

  1. Wiedlocha, A. and V. Sorensen (2004) Curr. Top. Microbiol. Immunol. 286:45.
  2. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
  3. de Lapeyriere, O. et al. (1990) Oncogene 5:823.
  4. Ornitz, D.M. et al. (1996) J. Biol. Chem. 271:15292.
  5. Pizette, S. et al. (1991) Cell Growth Differ. 2:561.
  6. Asada, M. et al. (1999) Growth Factors 16:293.
  7. Ropiquet, F. et al. (2000) Cancer Res. 60:4245.
  8. de Lapeyriere, O. et al. (1993) Development 118:601.
  9. Li, J. et al. (1993) Cancer 72:2253.
  10. Floss, T. et al. (1997) Genes Dev. 11:2040.
  11. Grass, S. et al. (1996) Development 122:141.

Long Name

Fibroblast Growth Factor 6

Alternate Names

FGF6, HBGF-6, HST2

Entrez Gene IDs

2251 (Human); 14177 (Mouse); 170700 (Rat)

Gene Symbol

FGF6

UniProt

NP_034334 (Mouse)

Additional FGF-6 Products

Product Documents for Recombinant Mouse FGF-6 Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 5750-F6 (with carrier)
Download SDS 5750-F6/CF (carrier free)

Product Specific Notices for Recombinant Mouse FGF-6 Protein

For research use only

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