Recombinant Mouse FGFR5 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 1899-FR

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
1899-FR-050
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

NS0

Accession #

NP_473412

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all FGFR5/FGFRL1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse FGF R5/FGFRL1 protein
Mouse FGF R5
(Ala21-Pro374)
Accession # NP_473412		 PIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala21

Predicted Molecular Mass

65.6 kDa (monomer)

SDS-PAGE

85-95 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.

200 ng/mL of biotinylated Recombinant Mouse FGF1 is mixed with serially diluted Recombinant Mouse FGF R5 Fc Chimera. Following incubation, the FGF R5-FGF1/biotin complex is captured on an EvenCoatTM Streptavidin Microplate (Catalog # CP003). Bound FGF R5 is measured using Rt x mFGF R5 followed by HRP labeled Gt x Rat IgG. The concentration of Recombinant Mouse FGF R5 Fc Chimera that produces 50% of the optimal binding is 30-120 ng/mL.

Scientific Data Images for Recombinant Mouse FGFR5 Fc Chimera Protein, CF

Recombinant Mouse FGFR5 Fc Chimera Protein Binding Activity

Recombinant Mouse FGFR5 Fc Chimera Protein Binding Activity

Recombinant Mouse FGF R5 Fc Chimera (Catalog # 1899-FR) binds biotinylated Recombinant Mouse FGF1. The concentration of Recombinant Mouse FGF R5 Fc Chimera that produces 50% of the optimal binding response is 30-120 ng/mL.

Formulation, Preparation and Storage

1899-FR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGFR5/FGFRL1

Fibroblast growth factor receptor 5 (FGF R5), also known as FGRL1, is a 65 kDa transmembrane member of the FGF receptor family (1). Mature mouse FGF R5 consists of a 354 amino acid (aa) extracellular domain (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 134 aa cytoplasmic domain (2). FGF R5 is distinct from other FGF receptor family members in that it does not contain a cytoplasmic tyrosine kinase domain (2, 3). Within the ECD, mouse FGF R5 shares 94% and 98% aa sequence identity with human and rat FGF R5, respectively. Alternate splicing results in an isoform that lacks the first Ig-like domain (2). FGF R5 is widely expressed, and expression of two species of mRNA in cartilage and pancreas may indicate the presence of both splice forms (2 - 6). Both the full length and truncated isoforms of FGF R5 bind FGF basic; the full length form additionally binds to heparin (2, 6). FGF R5 may function as a decoy receptor by binding FGF but not transducing its mitogenic signals (6).

References

  1. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
  2. Sleeman, M. et al. (2001) Gene 271:171.
  3. Kim, I. et al. (2001) Biochim. Biophys. Acta 1518:152.
  4. Antoine, M. et al. (2005) Growth Factors 23:87.
  5. Wiedemann, M. et al. (2000) Genomics 69:275.
  6. Trueb, B. et al. (2003) J. Biol. Chem. 278:33857.

Long Name

Fibroblast Growth Factor Receptor-like 1

Alternate Names

FGF R5, FGFRL1

Entrez Gene IDs

53834 (Human); 116701 (Mouse)

Gene Symbol

FGFRL1

UniProt

NP_473412

Additional FGFR5/FGFRL1 Products

Product Documents for Recombinant Mouse FGFR5 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Mouse FGFR5 Fc Chimera Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.