Recombinant Mouse FOLR2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6830-FR

R&D Systems, part of Bio-Techne
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6830-FR-050
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Key Product Details

Source

NS0

Accession #

Q05685

Conjugate

Unconjugated

Applications

Bioactivity

View all FOLR2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse FOLR2 protein
Met-Ser227, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg21

Predicted Molecular Mass

25.1 kDa

SDS-PAGE

30-37 kDa, reducing conditions

Activity

Measured by its ability to bind Folic Acid-Bovine Serum Albumin with an estimated Kd < 1.6 nM.

Formulation, Preparation and Storage

6830-FR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FOLR2

Folate Receptor 2 (FOLR2), also known as Folate Receptor beta, is a 38 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1, 2). Mature FOLR2 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage (3 - 5). Mouse FOLR2 shares 82% and 91% amino acid sequence identity with human and rat FOLR2, respectively. FOLR2 is predominantly expressed in placenta, cells of the neutrophilic lineage, and some CD34+ hematopoietic progenitor cells (4, 6, 7). It is up‑regulated on myeloid  leukemias, head and neck squamous cell carcinomas, and several nonepithelial cancers (3, 6, 8). It is also up‑regulated on immunosuppressive tumor-associated macrophages as well as on macrophages and monocytes at chronic inflammatory sites including rheumatoid arthritis synovium and glioblastoma (9 - 12). FOLR2 knockout mice do not show gross morphological defects, but they exhibit increased sensitivity to arsenate-induced teratogenicity (13, 14).

References

  1. Fowler, B. et al. (2001) Kidney Int. 59:S-221.
  2. Kelemen, L.E. (2006) Int. J. Cancer 119:243.
  3. Shen, F. et al. (1994) Biochemistry 33:1209.
  4. Ratnam, M. et al. (1989) Biochemistry 28:8249.
  5. Brigle, K.E. et al. (1991) J. Biol. Chem. 266:17243.
  6. Ross, J.F. et al. (1999) Cancer 85:348.
  7. Reddy, J.A. et al. (1999) Blood 93:3940.
  8. Ross, J.F. et al. (1994) Cancer 73:2432.
  9. Puig-Kroger, A. et al. (2009) Cancer Res. 69:9395.
  10. Nakashima-Matsushita, N. et al. (1999) Arthritis Rheum. 42:1609.
  11. van der Heijden, J.W. et al. (2009) Arthritis Rheum. 60:12.
  12. Nagai, T. et al. (2009) Cancer Immunol. Immunother. 58:1577.
  13. Piedrahita, J.A. et al. (1999) Nat. Genet. 23:228.
  14. Wlodarczyk, B. et al. (2001) Toxicol. Appl. Pharmacol. 177:238.

Long Name

Folate Receptor 2

Alternate Names

beta-HFR, FR-beta, FR-P3

Entrez Gene IDs

2350 (Human); 14276 (Mouse); 293154 (Rat)

Gene Symbol

FOLR2

UniProt

Q05685

Additional FOLR2 Products

Product Documents for Recombinant Mouse FOLR2 Protein, CF

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Product Specific Notices for Recombinant Mouse FOLR2 Protein, CF

For research use only

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