Recombinant Mouse Frizzled-10 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9247-FZ

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
9247-FZ-050
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

NS0

Accession #

Q8BKG4

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Frizzled-10 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Frizzled-10 protein
Mouse Frizzled-10
(Ile22-Phe166)
Accession # Q8BKG4		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile22

Predicted Molecular Mass

44 kDa

SDS-PAGE

53-61 kDa, reducing conditions

Activity

Measured by its ability to bind biotinylated recombinant mouse Wnt-3a in a functional ELISA with an ED50 of 10-60 ng/mL.

Scientific Data Images for Recombinant Mouse Frizzled-10 Fc Chimera Protein, CF

Recombinant Mouse Frizzled-10 Fc Chimera Protein Bioactivity

Recombinant Mouse Frizzled-10 Fc Chimera Protein Bioactivity

Recombinant Mouse Frizzled-10 Fc Chimera (Catalog # 9247-FZ) binds to Biotinylated Recombinant Mouse Wnt-3a in an ELISA Binding Assay. When Biotinylated Recombinant Mouse Wnt-3a is coated on a streptavidin coated ELISA plate, Recombinant Mouse Frizzled-10 Fc Chimera binds with an ED50 = 10-60 ng/mL.

Formulation, Preparation and Storage

9247-FZ
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-10

Frizzled-10, also known as CD350, is a 68 kDa seven pass transmembrane glycoprotein in the frizzled family of Wnt receptors (1, 2). The N-terminal extracellular region of Frizzled-10 contains a cysteine-rich domain that comprises the Wnt binding domain and mediates receptor oligomerization (3-5). The C-terminal cytoplasmic tail contains a PDZ-interaction motif (3). Within the cysteine-rich domain, human Frizzled-10 shares 94% and 99% aa sequence identity with human and rat Frizzled-10, respectively. Frizzled-10 is expressed during embryogenesis in the primitive streak, dorsal neural tube, developing brain, limb bud, and airway epithelium (6-12). It is induced by Shh and colocalizes with Shh and Wnt-7a in the neural tube (13, 14). In the adult, Frizzled-10 is expressed in placenta, gastric glands, and colon and renal tubule epithelial cells (4). Frizzled-10 associates with LRP5 and transduces Wnt-1, -7a, -7b, and -8 signals, resulting in the stabilization of cytoplasmic beta-Catenin (11, 12, 14). It is important for the differentiation of sensory neurons (12). Frizzled-10 is also up-regulated in some cancers and transformed cell lines (4, 15). It binds hypoxia inducible gene 2, which promotes oncogenic Wnt signaling and functions as an autocrine growth factor for renal cell carcinomas (16).

References

  1. Kikuchi, A. et al. (2007) Cell. Signal. 19:659.
  2. Wang, H. et al. (2006) Cell. Signal. 18:934.
  3. Koike, J. et al. (1999) Biochem. Biophys. Res. Commun. 262:39.
  4. Nagayama, S. et al. (2005) Oncogene 24:6201.
  5. Dann, C.E. et al. (2001) Nature 412:86.
  6. Kemp, C.R. et al. (2007) Dev. Dyn. 236:2011.
  7. Kawakami, Y. et al. (2000) Mech. Dev. 91:375.
  8. Nasevicius, A. et al. (2000) Mech. Dev. 92:311.
  9. Moriwaki, J. et al. (2000) Biochem. Biophys. Res. Commun. 278:377.
  10. Stark, M.R. et al. (2000) Mech. Dev. 93:195.
  11. Wang, Z. et al. (2005) Mol. Cell. Biol. 25:5022.
  12. Garcia-Morales, C. et al. (2009) Dev. Biol. 335:143.
  13. Nunnally, A.P. and B.A. Parr (2004) Dev. Genes Evol. 214:144.
  14. Kawakami, Y. et al. (2000) Dev. Growth Differ. 42:561.
  15. Saitoh, T. et al. (2002) Int. J. Oncol. 20:117.
  16. Togashi, A. et al. (2005) Cancer Res. 65:4817.

Alternate Names

CD350, Frizzled10, FZD10

Entrez Gene IDs

11211 (Human); 93897 (Mouse); 64512 (Rat)

Gene Symbol

FZD10

UniProt

Q8BKG4

Additional Frizzled-10 Products

Product Documents for Recombinant Mouse Frizzled-10 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Mouse Frizzled-10 Fc Chimera Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.