Recombinant Mouse Galectin-2 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 8579-GA
Key Product Details
Product Specifications
Source
Human embryonic kidney cell, HEK293-derived mouse Galectin-2 protein
Ser2-Glu130
Ser2-Glu130
Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ser2
Predicted Molecular Mass
15 kDa
SDS-PAGE
12-14 kDa, reducing conditions
Activity
Measured by the ability of the immobilized protein to support the adhesion of EOMA mouse endothelial cells.
The ED50 for this effect is 0.5-2.5 μg/mL.
Measured by its ability to agglutinate human red blood cells. Hadari, Y.R. et al. (2000) J. Cell Sci. 113:2385.
The ED50 for this effect is 2.5-12.5 μg/mL.
The ED50 for this effect is 0.5-2.5 μg/mL.
Measured by its ability to agglutinate human red blood cells. Hadari, Y.R. et al. (2000) J. Cell Sci. 113:2385.
The ED50 for this effect is 2.5-12.5 μg/mL.
Formulation, Preparation and Storage
8579-GA
| Formulation | Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl, EDTA, DTT and Trehalose. |
| Reconstitution |
Reconstitute at 400 μg/mL in water.
|
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Galectin-2
Galectin-2 is an approximately 14 kDa, homodimeric carbohydrate-binding protein of the S-type lectin family (1, 2). Like other prototype Galectins, Galectin-2 consists of a single carbohydrate-recognition domain (CRD) (3). Mouse Galectin-2 shares 86% and 66% amino acid sequence identity with rat and human Galectin-2, respectively. Galectin-2 is primarily expressed in the digestive tract and skin of mice (4-6). It can be S-nitrosylated with S-nitrosoglutathione in mouse gastric mucosa (7).
S-nitrosylation prevents Galectin-2 inactivation by hydrogen peroxide, suggesting that this modification protects Galectin-2 function against oxidative stress (8). Galectin-2 appears to have a role in immune responses. Galectin-2 mRNA and protein levels are down-regulated following infection with H. felis (9). Additionally, Galectin-2 treatment has been shown to reduce MHC class I-restricted immune responses and ameliorate acute and chronic experimental colitis in mice by inducing apoptosis of T cells (6, 10). Galectin-2 may also play a role in cancer since circulating levels of human Galectin-2 are elevated in colon and breast cancer patients (11).
References
- Hokama, A. et al. (2008) World J. Gastroenterol. 14:5133.
- Barondes, S.H. et al. (1994) Cell 76:597.
- Hirabayashi, J. and K. Kasai (1993) Glycobiology 3:297.
- Nio, J. et al. (2005) J. Histochem. Cytochem. 53:1323.
- Nio-Kobayashi, J. et al. (2009) J. Histochem. Cytochem. 57:41.
- Loser, K. et al. (2009) J. Immunol. 182:5419.
- Ohtake, K. et al. (2009) J. Proteomics 72:750.
- Tamura, M. et al. (2015) Biochem. Biophys. Res. Commun. 457:712.
- Takaishi, S. and T.C. Wang (2007) Cancer Sci. 98:284.
- Paclik, D. et al. (2008) J. Mol. Med. 86:1395.
- Chen, C. et al. (2014) Br. J. Cancer 110:741.
Alternate Names
GAL2, Galectin2, LGALS2
Gene Symbol
LGALS2
UniProt
Additional Galectin-2 Products
Product Documents for Recombinant Mouse Galectin-2 Protein, CF
Product Specific Notices for Recombinant Mouse Galectin-2 Protein, CF
For research use only
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