Recombinant Mouse GDF-6/BMP-13 Protein
R&D Systems, part of Bio-Techne | Catalog # 855-G6
Key Product Details
Source
E. coli
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
E. coli-derived mouse GDF-6/BMP-13 protein
Thr335-Arg454
Thr335-Arg454
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Thr335
Predicted Molecular Mass
13.7 kDa (monomer)
Activity
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351.
The ED50 for this effect is typically 0.85-5 µg/mL.
The ED50 for this effect is typically 0.85-5 µg/mL.
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 855-G6
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 300 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 855-G6/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 300 μg/mL in sterile 4 mM HCl. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: GDF-6/BMP-13
Growth Differentiation Factor 6 (GDF-6), also known as bone morphogenetic protein 13 (BMP-13) or cartilage-derived morphogenetic protein 2 (CDMP-2), is a member of the bone morphogenetic protein (BMP) family which belongs to the transforming growth factor beta (TGF-beta) superfamily. The mature GDF-6 with 120 amino acids is a homodimeric protein containing the characteristic seven conserved cysteine residues. GDF-5, GDF-6 and GDF-7, which share 80-86% identity, define a new subgroup within the BMP family. Like other TGF-beta superfamily proteins, GDF-6 is highly conserved across species. At the amino acid sequence level, human and mouse GDF-6 are 99% identical. It has been reported that GDF-6 has multiple functions including regulation of myogenesis, regulation of chondrogenesis, bone morphogenesis, and neuron differentiation and survival. GDF-6 response is mediated by the formation of hetero-oligomeric complexes of type I (BMPR-IB) and type II (BMPR-II or Activin R-II) sereine/threonine kinase receptors, and the activation of Smad proteins (Smad 1, 5, and 8).
References
- Storm, E.E. et al. (1994) Nature 368:639.
- Nishitoh, H. et al. (1996) J. Biol. Chem. 271:21345.
- Francis-West, P.H. et al. (1999) Development 126:1035.
- Massague, J. et al. (2000) Genes and Dev. 14:627.
- Inada, M. et al. (1996) BBRC, 222:317.
- Settle, S.H., Jr. et al. (2003) Dev. Biol. 254:116.
Long Name
Growth Differentiation Factor 6
Alternate Names
BMP-13, GDF6
Gene Symbol
GDF6
UniProt
Additional GDF-6/BMP-13 Products
Product Documents for Recombinant Mouse GDF-6/BMP-13 Protein
Product Specific Notices for Recombinant Mouse GDF-6/BMP-13 Protein
For research use only
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