Recombinant Mouse GITR/TNFRSF18 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 524-GR

R&D Systems, part of Bio-Techne
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524-GR-050
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Key Product Details

Source

NS0

Accession #

Q8C4K3

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity

View all GITR/TNFRSF18 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse GITR/TNFRSF18 protein
Mouse GITR
(Ser22-His153)
Accession # Q8C4K3		 IEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser22

Predicted Molecular Mass

42 kDa (monomer)

SDS-PAGE

55-65 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized rmGITR/Fc Chimera at 2 µg/mL (100 µL/well) can bind rmGITR Ligand with a linear range of 0.8-50 ng/mL.

Reviewed Applications

Read 4 reviews rated 4.5 using 524-GR in the following applications:

Formulation, Preparation and Storage

524-GR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GITR/TNFRSF18

GITR (glucocorticoid-induced tumor necrosis factor receptor), also known as AITR and TNFRSF18, is a 40 kDa transmembrane glycoprotein that functions in immune regulation (1, 2). Mature mouse GITR consists of a 134 amino acid (aa) extracellular domain (ECD) with three tandem TNFR cysteine-rich repeats, a 21 aa transmembrane segment, and a 55 aa cytoplasmic domain (3). Within the ECD, mouse GITR shares 55% and 86% aa sequence identity with human and rat GITR, respectively. Alternative splicing generates additional isoforms with substituted cytoplasmic domains or with a substitution within the third TNFR repeat, transmembrane, and cytoplasmic regions (4). GITR is expressed on CD4+CD25+ regulatory T cells (Treg) as well as on subsets of thymocytes, lymph node cells, and splenocytes (3, 5, 6), and it is upregulated on antigen-activated conventional CD4+ and CD8+ T cells (3, 6-8). GITR binding by GITR Ligand/TNFSF18 costimulates the proliferation and activation of CD4+ or CD8+ conventional T cells (7-10). It also induces the proliferation of Treg (9, 11) but inhibits the ability of Treg to suppress immune responses (5, 9, 12-14). This can result in the development of autoimmunity, increased tumor cell killing by effector T cells (5, 12), and increased inflammation in arthritis, allergic asthma, and inflammatory bowel disease (11, 15). GITR is also expressed on sympathetic neurons where it enhances NGF-induced neurite outgrowth and branching (16).

References

  1. Clouthier, D.L. and T.H. Watts (2014) Cytokine Growth Factor Rev. 25:91.
  2. Ronchetti, S. et al. (2015) J. Immunol. Res. 2015:171520.
  3. Nocentini, G. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6216.
  4. Nocentini, G. et al. (2000) Cell Death Differ. 7:408.
  5. Shimizu, J. et al. (2002) Nat. Immunol. 3:135.
  6. Kwon, B. et al. (1999) J. Biol. Chem. 274:6056.
  7. Gurney, A.L. et al. (1999) Curr. Biol. 9:215.
  8. Muriglan, S.J. et al. (2004) J. Exp. Med. 200:149.
  9. Ronchetti, S. et al. (2004) Eur. J. Immunol. 34:613.
  10. Tone, M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:15059.
  11. Ephrem, A. et al. (2013) Eur. J. Immunol. 43:2421.
  12. Ko, K. et al. (2005) J. Exp. Med. 202:885.
  13. Ji, H. et al. (2004) J. Immunol. 172:5823.
  14. Stephens, G.L. et al. (2004) J. Immunol. 173:5008.
  15. Patel, M. et al. (2005) Eur. J. Immunol. 35:3581.
  16. O'Keeffe, G.W. et al. (2008) Nat. Neurosci. 11:135.

Long Name

Glucocorticoid Induced TNF Receptor Family Related Gene

Alternate Names

AITR, CD357, TNFRSF18

Entrez Gene IDs

8784 (Human); 21936 (Mouse); 500598 (Rat); 102146362 (Cynomolgus Monkey)

Gene Symbol

TNFRSF18

UniProt

Q8C4K3

Additional GITR/TNFRSF18 Products

Product Documents for Recombinant Mouse GITR/TNFRSF18 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse GITR/TNFRSF18 Fc Chimera Protein, CF

For research use only

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