Recombinant Mouse Granzyme D Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1365-SE
Key Product Details
Product Specifications
Source
Ile21-Leu252 with a C-terminal 10-His tag
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
The specific activity is >50 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
1365-SE
Formulation | Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl. |
Reconstitution | Reconstitute at 100 μg/mL in sterile 25 mM HEPES and 200 mM NaCl, pH 7.5. |
Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Granzyme D
Granzyme D is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1-3). Granzyme D’s functions are largely unknown. However, its selective expression in primary and functional hematopoietic stromal lines correlates with capacity of supporting growth of a pre-T lymphoma clone (4). Together with Granzymes E, F and G, it is regulated through pregnancy and by IL-2 and IL-15 in granulated metrial gland cells (5). The human or rat counterpart of mouse Granzyme D has not been found. Like other granzymes, mouse Granzyme D is not secreted as a zymogen but stored as a fully processed and activated enzyme in the cytoplasmic granules of CTL (1). It is synthesized as a 252 amino acid precursor with an 18 amino acid signal peptide and a 2 amino acid propeptide (1). The mature protein (residues 21-252) is expressed and purified.
References
- Jenne, D.E. et al. (1988) Proc. Natl. Acad. Sci. USA 85:4814.
- Kam, C.-M. et al. (2000) Biochim. Biophys. Acta 1477:307.
- Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
- Yokoi, A. et al. (1999) Biochem. Biophys. Res. Comm. 264:768.
- Allen, M.P. and M. Nilsen-Hamilton (1998) J. Immunol. 161:2772.
Alternate Names
Entrez Gene IDs
Gene Symbol
UniProt
Additional Granzyme D Products
Product Documents for Recombinant Mouse Granzyme D Protein, CF
Product Specific Notices for Recombinant Mouse Granzyme D Protein, CF
For research use only