Skip to main content

Recombinant Mouse ICAM-2/CD102 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 774-M2

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
774-M2-050
774-M2-200

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse ICAM-2/CD102 protein
Mouse ICAM-2
(Ser20-Gln222)
Accession # P35330
DIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser20

Predicted Molecular Mass

49.5 kDa (monomer)

SDS-PAGE

75-80 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells.
Recombinant Mouse ICAM‑2/CD102 Fc Chimera immobilized at 12.5 µg/mL (100 µL/well) will induce >65% cell adhesion.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

774-M2
Formulation Lyophilized from a 0.2 μm filtered solution in Tris, Citric Acid and NaCl.
Reconstitution
Reconstitute at 0.4 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Loading...
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-2/CD102

Intercellular Adhesion Molecule-2 (ICAM-2, CD102), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). ICAM-2 is constitutively expressed at high levels on vascular endothelial cells and lymphohematopoietic cells. ICAM-2 mediated adhesion has been shown to provide a co-stimulatory signal for T cell aggregation, NK cytotoxicity and NK cell migration. The mouse and human ICAM-2 proteins share 60% amino acid identity. Human LFA-1 binds equally well to human or mouse ICAM-2.

References

  1. Somersalo, K. et al. (1995) J. Biol. Chem. 270:8629.
  2. Hayflick, J. et al. (1998) Immunologic Res. 17:313.
  3. Xu, H. (1992) J. Immunol. 149:2650.

Long Name

Intercellular Adhesion Molecule 2

Alternate Names

CD102, ICAM2

Entrez Gene IDs

3384 (Human); 15896 (Mouse)

Gene Symbol

ICAM2

UniProt

Additional ICAM-2/CD102 Products

Product Documents for Recombinant Mouse ICAM-2/CD102 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse ICAM-2/CD102 Fc Chimera Protein, CF

For research use only

Loading...
Loading...
Loading...