Recombinant Mouse IFN-alpha 7/IFNA7 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10208-IJ

R&D Systems, part of Bio-Techne
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10208-IJ-010
10208-IJ-050
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Key Product Details

Source

CHO

Accession #

P06799

Conjugate

Unconjugated

Applications

Bioactivity

View all IFN-alpha 7/IFNA7 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse IFN-alpha 7/IFNA7 protein
Cys24-Glu190

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Predicted Molecular Mass

19 kDa

SDS-PAGE

18-24 kDa, under reducing conditions

Activity

Measured in an anti-viral assay using L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681.
The ED50 for this effect is <1.5 ng/mL.

Scientific Data Images for Recombinant Mouse IFN-alpha 7/IFNA7 Protein, CF

Recombinant Mouse IFN-alpha 7/IFNA7 Protein Bioactivity

Recombinant Mouse IFN-alpha 7/IFNA7 Protein Bioactivity

Recombinant Mouse IFN-alpha 7/IFNA7 (Catalog # 10208-IJ) suppresses viral activity on L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is <1.5 ng/mL.
Recombinant Mouse IFN-alpha 7/IFNA7 Protein SDS-PAGE

Recombinant Mouse IFN-alpha 7/IFNA7 Protein SDS-PAGE

2 μg/lane of Recombinant Mouse IFN-alpha J1/IFNA7 (Catalog # 10208-IJ) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18-24 kDa.

Formulation, Preparation and Storage

10208-IJ
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha 7/IFNA7

Interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, classified based on their binding specificity to cell surface receptors (1). There are more than a dozen closely related IFN alpha subtypes found in both the human and mouse genome, each sharing about 80% amino acid (aa) sequence homology (2, 3). Mature mouse IFNA7 consists of 166 aa and shares 59% identity with human IFNA7. The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 (alpha -subunit) and IFNAR2 (beta -subunit) (4, 5). Individual IFN alpha subtypes are known to display unique efficacies to viral protection, with IFNA7 showing slightly lower antiviral potency against Mengo virus but maintaining similar anti-proliferative potency in B16 melanoma cells (5, 6).

References

  1. Pestka S, et al. (1987) Annu Rev Biochem. 56:727.
  2. Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
  3. Schreiber, G. and J. Piehler (2015) Trends Immunol. 36:139.
  4. Fung, K.Y. et al. (2013) Science 339:1088.
  5. van Pesch, V. et al. (2004) J. Virol. 78:8219.
  6. James CM et al. (2007) Vaccine. 25(10):1856.

Long Name

Interferon alpha 7

Alternate Names

IFN-alpha J1, IFNA7, IFNalpha J1

Entrez Gene IDs

3444 (Human); 15970 (Mouse)

Gene Symbol

IFNA7

UniProt

P06799

Additional IFN-alpha 7/IFNA7 Products

Product Documents for Recombinant Mouse IFN-alpha 7/IFNA7 Protein, CF

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