Recombinant Mouse IGSF2/CD101 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 3368-CD

R&D Systems, part of Bio-Techne
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3368-CD-050
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Key Product Details

Source

NS0

Accession #

A8E0Y8

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all IGSF2/CD101 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse IGSF2/CD101 protein
Mouse CD101
(Gln21-Phe974)
Accession # A8E0Y8		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln21 inferred from enzymatic pyroglutamate treatment revealing Arg22.

Predicted Molecular Mass

133 kDa

SDS-PAGE

121-150 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells.
The ED50 for this effect is 1.4-8.4 μg/mL.

Scientific Data Images for Recombinant Mouse IGSF2/CD101 Fc Chimera Protein, CF

Recombinant Mouse IGSF2/CD101 Fc Chimera Protein Bioactivity

Recombinant Mouse IGSF2/CD101 Fc Chimera Protein Bioactivity

Recombinant Mouse IGSF2/CD101 Fc Chimera (Catalog # 3368-CD) inhibits anti-CD3 antibody induced human T cell proliferation. The ED50 for this effect is 1.4-8.4 μg/mL.
Recombinant Mouse IGSF2/CD101 Fc Chimera Protein SDS-PAGE

Recombinant Mouse IGSF2/CD101 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Mouse IGSF2/CD101 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 121-150 kDa and 240-280 kDa, respectively.

Formulation, Preparation and Storage

3368-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: IGSF2/CD101

CD101, also known as Immunoglobulin Superfamily member 2 (IGSF2) or cell surface glycoprotein V7 (V7), is a 130-145 kDa type 1 transmembrane glycoprotein within the very large Ig superfamily (1). Ig superfamily members participate in diverse functions including cell-cell recognition and adhesion, soluble molecules and specific antigen recognition, and are grouped based on the presence of a characteristic Ig domain fold. Mature mouse CD101 consists of a large extracellular domain (ECD) containing a Glu-Trp-Ile (EWI) motif and 7 Ig-like V-type domains, a single transmembrane domain, and a short cytoplasmic domain (1, 2). The mouse CD101 ECD shares 71% and 87% amino acid sequence identity with human and rat CD101 ECD, respectively. The exact biological functions, interacting ligands and mode of signal transduction for CD101 currently remain unclear. CD101 expression has been found on dermal dendritic cells (DCs), granulocytes, monocytes, activated T cells, and CD4+ CD25+ FoxP3+ T regulatory cells (Tregs) (2-5). CD101 expression has been linked to immune suppression by Tregs in humans (6), with ligation of CD101 on DCs inducing IL-10 expression (2) and ligation on activated T cells blocking IL-2 expression (7). Both of these effects down-regulate T cell activity. Notably, in mice, CD101 expression on CD62L++ Tregs identifies a population of cells that have potent suppressor activity (5). In humans, reduced CD101 expression on mucosal CD8+ T cells has been linked to increased tissue inflammation in both intestinal (8) and pulmonary mucosa (9). In both mouse and human, mutations in CD101 have been associated with increased susceptibility for diabetes (10, 11). CD101 expression may act as a potential biomarker for CD4+ CD56+ blastic tumors (12) and inflammatory bowel disease activity (13), and missense variants of its gene have been associated with increased risk of HIV-1 acquisition (14).

References

  1. Stipp, C. et al. (2001) J. Biol. Chem. 276:40545.
  2. Bouloc, A. et al. (2000) Eur. J. Immunol. 30:3132.
  3. Rivas, A. et al. (1995) J. Immunol. 154:4423.
  4. Ruegg, C. et al. (1995) J. Immunol. 154:4434-CD.
  5. Fernandez, I. et al. (2007) J. Immunol. 179:2808.
  6. Hua, J. et al. (2015) J. Immunol. 195:3642.
  7. Soares, L. et al. (1998) J. Immunol. 161:209.
  8. Brimnes, J. et al. (2005) J. Immunol. 174:5814.
  9. Kumar, B. et al. (2017) Cell Rep. 20:2921.
  10. Rainbow, D. et al. (2011) J Immunol. 187:325.
  11. Okuno, M. et al. (2017) J Diabetes Investig. 8:286.
  12. Meyer, N. et al. (2005) J. Invest. Dermatol. 124:668.
  13. Schey, R. et al. (2016) Mucosal Immunol. 9:1205.
  14. Mackelprang, R. et al. (2017) PLoS Pathog. 13:e1006703.

Long Name

Immunoglobulin Superfamily, Member 2

Alternate Names

CD101, Leukocyte Surface Protein, V7

Entrez Gene IDs

9398 (Human); 630146 (Mouse)

Gene Symbol

CD101

UniProt

A8E0Y8

Additional IGSF2/CD101 Products

Product Documents for Recombinant Mouse IGSF2/CD101 Fc Chimera Protein, CF

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