Recombinant Mouse IGSF9 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9140-S9

R&D Systems, part of Bio-Techne
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9140-S9-050
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Key Product Details

Source

NS0

Accession #

Q05BQ1

Conjugate

Unconjugated

Applications

Bioactivity

View all IGSF9 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse IGSF9 protein
Arg21-Leu730, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg21

Predicted Molecular Mass

78 kDa

SDS-PAGE

95-110 kDa, reducing conditions

Activity

Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons.
Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 150 ng on a nitrocellulose-coated microplate

Scientific Data Images for Recombinant Mouse IGSF9 Protein, CF

Recombinant Mouse IGSF9 Protein Bioactivity

Recombinant Mouse IGSF9 Protein Bioactivity

Recombinant Mouse IGSF9 (Catalog # 9140-S9) Inhibits Neurite Outgrowth in E13 Chicken Embryonic Dorsal Root Ganglia (DRG) Neurons. A) Normal Neurite outgrowth is observed in untreated chicken E13 DRG neurons. B) Chicken DRG neurite outgrowth is significantly inhibited when Recombinant Mouse IGSF9 is immobilized as a 3 μL droplet containing 150 ng of IGSF9 protein on a nitrocellulose-coated microplate.

Formulation, Preparation and Storage

9140-S9
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGSF9

IGSF9, also known as Dasm1, is an approximately 130 kDa transmembrane protein that plays a role in neuronal synapse maintenance and function (1). Mature mouse Dasm1 consists of a 714 amino acid (aa) extracellular domain (ECD) with 5 Ig-like domains and 2 fibronectin type-3 domains, a 21 aa transmembrane segment, and a 424 aa cytoplasmic domain (2). Within the ECD, mouse Dasm1 shares 90% and 96% aa sequence identity with human and rat Dasm1, respectively. Alternative splicing generates an additional isoform that is truncated following the fifth Ig-like domain. Dasm1 is expressed in the dorsal root and trigeminal ganglia, forebrain, cortex, dentate gyrus, pyramidal cells, Purkinje cells, and hippocampal CA1 interneurons (2-5). It localizes to dendrites, cell bodies, and post-synaptic densities (3, 6). Dasm1 functions as a homophilic adhesion protein that supports the maintenance of inhibitory synapses as well as inhibitory neurotransmission (5, 6).

References

  1. Hansen, M. and P.S. Walmod (2013) Neurochem. Res. 38:1236.
  2. Doudney, K. et al. (2002) Genomics 79:663.
  3. Shi, S.-H. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13341.
  4. Mishra, A. et al. (2008) Mol. Cell. Biol. 28:2782.
  5. Mishra, A. et al. (2014) J. Neurosci. 34:4187.
  6. Shi, S.-H. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13346.

Long Name

Immunoglobulin Superfamily, Member 9

Alternate Names

Dasm1, FP18798, IGSF9A, KIAA1355, Nrt1, Protein Turtle Homolog A, TUTLA

Entrez Gene IDs

57549 (Human); 93842 (Mouse); 304982 (Rat)

Gene Symbol

IGSF9

UniProt

Q05BQ1

Additional IGSF9 Products

Product Documents for Recombinant Mouse IGSF9 Protein, CF

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Product Specific Notices for Recombinant Mouse IGSF9 Protein, CF

For research use only

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