Recombinant Mouse IL-17RE Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7997-MR

R&D Systems, part of Bio-Techne
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7997-MR-025
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Key Product Details

Source

NS0

Accession #

Q8BH06

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-17RE Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse IL-17 RE protein
Mouse IL-17 RE
(Ala115-His414)
Accession # Q8BH06		 IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala115

Predicted Molecular Mass

61 kDa (monomer)

SDS-PAGE

65-70 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse IL-17 RE Fc Chimera is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse IL‑17C (Catalog # 2306-ML) that produces 50% of the optimal binding response is approximately 0.4-2.4 ng/mL.

Formulation, Preparation and Storage

7997-MR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17RE

Interleukin‑17 Receptor E (IL‑17 RE) is an approximately 70 kDa (predicted) transmembrane protein in the family of IL‑17 receptors. IL‑17 RE is required for mediating the pro‑inflammatory and homeostatic actions of IL‑17C in the skin and mucosa (1, 2). Mature mouse IL‑17 RE consists of a 391 amino acid (aa) extracellular domain, a 21 aa transmembrane segment, and a 202 aa cytoplasmic domain with one SEFIR/TIR domain (3). Within aa 115‑414, mouse IL‑17 RE shares 79% and 90% aa sequence identity with human and rat IL‑17 RE, respectively. Alternative splicing of mouse IL‑17 RE generates additional isoforms with either 201 aa or 326 aa N‑terminal deletions or deletion/substitution of the transmembrane segment (3). IL‑17 RE is expressed on keratinocytes, mucosal epithelial cells, Th17 cells, and gamma/ delta T cells (4, 5). It associates with the widely expressed IL‑17 RA to form a heterodimeric receptor for IL-17C (4‑6). IL-17C binds to IL‑17 RE with high affinity and to IL‑17 RA with low affinity (4, 5). IL‑17C expression is induced by inflammatory stimulation in colon and airway epithelial cells, keratinocytes, CD4+ T cells, macrophages, and dendritic cells (4, 6, 7‑9). It is up‑regulated in various chronic inflammatory diseases including psoriasis, cystic fibrosis, and chronic obstructive pulmonary disease (COPD) (7, 8, 10). IL‑17 RE is reciprocally down‑regulated in psoriatic lesions (10). The interaction of IL‑17C with IL‑17 RE promotes mucosal immunity through the induction of anti‑bacterial peptides and pro‑inflammatory cytokines and chemokines (4, 6, 8, 9). IL‑17C action supports the integrity of the colon epithelium following infection induced damage (4, 6, 11) but also contributes to psoriatic skin thickening and the progression of arthritis (4, 8, 9). IL‑17C is additionally up‑regulated in Th17 cell dependent autoimmunity (5). In this setting, it exacerbates disease severity by inducing Th17 cell production of IL‑17A, IL‑17F, IL‑22, CCR6, and CCL20 (5). The up‑regulation of IL‑17 RE in hepatocellular carcinoma is associated with poor prognosis (12).

References

  1. Pappu, R. et al. (2012) Trends Immunol. 33:343.
  2. Rubino, S.J. et al. (2012) Trends Immunol. 33:112.
  3. Li, T.S. et al. (2006) Cell. Signal. 18:1287.
  4. Ramirez-Carrozzi, V. et al. (2011) Nat. Immunol. 12:1159.
  5. Chang, S.H. et al. (2011) Immunity 35:611.
  6. Song, X. et al. (2011) Nat. Immunol. 12:1151.
  7. Pfeifer, P. et al. (2013) Am. J. Respir. Cell Mol. Biol. 48:415.
  8. Johnston, A. et al. (2013) J. Immunol. 190:2252.
  9. Yamaguchi, Y. et al. (2007) J. Immunol. 179:7128.
  10. Johansen, C. et al. (2009) Br. J. Dermatol. 160:319.
  11. Reynolds, J.M. et al. (2012) J. Immunol. 189:4226.
  12. Liao, R. et al. (2013) J. Exp. Clin. Cancer Res. 32:3.

Long Name

Interleukin 17 Receptor E

Alternate Names

IL-17 RE, IL17RE, Il25r

Entrez Gene IDs

132014 (Human); 57890 (Mouse); 362417 (Rat)

Gene Symbol

IL17RE

UniProt

Q8BH06 (Mouse)

Additional IL-17RE Products

Product Documents for Recombinant Mouse IL-17RE Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse IL-17RE Fc Chimera Protein, CF

For research use only

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