Recombinant Mouse IL-22BP Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 2376-BP

R&D Systems, part of Bio-Techne
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2376-BP-025
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Key Product Details

Source

NS0

Accession #

Q08AV1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity

View all IL-22BP Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse IL-22BP protein
Mouse IL-22BP
(Thr19-Pro230)
Accession # Q08AV1		 IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr19

Predicted Molecular Mass

51.8 kDa (monomer)

SDS-PAGE

60-70 kDa, reducing conditions

Activity

Measured by its ability to inhibit IL-22-induced IL-10 secretion by COLO 205 human colorectal adenocarcinoma cells.
The ED50 for this effect is 1-4 μg/mL in the presence of 0.5 ng/mL of Recombinant Mouse IL-22 (Catalog # 582-ML).

Formulation, Preparation and Storage

2376-BP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-22BP

Interleukin 22 binding protein (IL-22BP), also known as CRF2-10, CRF2-X, and IL-22 RA2, is a 35-45 kDa secreted glycoprotein in the type II cytokine receptor family (CRF). IL-22 signals through a receptor complex consisting of IL-22 R and IL-10 R beta. IL-10 R beta is also a component of the receptor complexes for IL-10, IL-26, IL-28, and IL-29 (1, 2). IL-22BP blocks the interaction of IL-22 with IL-22 R, preventing IL-22 induced production of reactive oxygen species, IL-6, IL-10, and TNF-a (3-8). In vivo, it regulates the proinflammatory effects of IL-22 (e.g. neutrophil infiltration) but not of IL-10 (7). Mouse IL-22BP can neutralize the bioactivity of both mouse and human IL-22 (6). IL-22BP is produced by dendritic cells (DE), epithelial cells, activated B cells, and activated monocytes (3, 6, 9, 10). It is constitutively expressed by DC but is down-regulated during local inflammation and in response to tissue damage (11-13). IL-22BP is critical for limiting IL-22 induced epithelial cell proliferation during wound healing, and its deficiency can enable uncontrolled proliferation and enhance tumor development (12). Mature mouse IL-22BP consists of two tandem Fibronectin type-III domains and shares 85% and 68% amino acid sequence identity with rat IL-22BP and the short isoform of human IL-22BP, respectively (6, 14).

References

  1. Lim, C. and R. Savan (2014) Cytokine Growth Factor Rev. 25:257.
  2. Mizoguchi, A. (2012) Inflamm. Bowel Dis. 18:1777.
  3. Xu, W. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9511.
  4. Kotenko, S.V. et al. (2001) J. Immunol. 166:7096.
  5. Li, J. et al. (2004) Int. Immunopharmacol. 4:693.
  6. Wei, C.-C. et al. (2003) Genes Immun. 4:204.
  7. Weber, G.F. et al. (2007) Infect. Immun. 75:1690.
  8. Whittington, H.A. et al. (2004) Am. J. Respir. Cell Mol. Biol. 31:220.
  9. Lecart, S. et al. (2002) Int. Immunol. 14:1351.
  10. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577.
  11. Wolk, K. et al. (2007) J. Immunol. 178:5973.
  12. Huber, S. et al. (2012) Nature 491:259.
  13. Martin, J.C.J. et al. (2014) Mucosal Immunol. 7:101.
  14. Weiss, B. et al. (2004) Genes Immun. 5:330.

Long Name

Interleukin 22 Binding Protein

Alternate Names

CRF2-10, CRF2-X, IL-22 RA2, IL22BP, IL22RA2

Entrez Gene IDs

116379 (Human); 237310 (Mouse)

Gene Symbol

IL22RA2

UniProt

Q08AV1

Additional IL-22BP Products

Product Documents for Recombinant Mouse IL-22BP Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse IL-22BP Fc Chimera Protein, CF

For research use only

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