Recombinant Mouse IL-24 (NS0-expressed) Protein

R&D Systems, part of Bio-Techne | Catalog # 7807-ML

R&D Systems, part of Bio-Techne
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Carrier Free
7807-ML-010/CF
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With Carrier
7807-ML-010
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Key Product Details

Source

NS0

Accession #

NP_444325

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-24 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse IL-24 protein
Gln66-Leu220

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln66 predicted: No results obtained, sequencing might be blocked

Predicted Molecular Mass

18 kDa (monomer)

SDS-PAGE

19‑23 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with human IL-20 R alpha and human IL-20 R beta.
The ED50 for this effect is 0.07-0.35 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 7807-ML
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 7807-ML/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-24

Interleukin 24 (IL-24), also known as FISP (IL-4 induced secreted protein) in mouse, and mda-7 (melanoma differentiation associated gene‑7) in humans, is a member of the IL-10 family of helical cytokines (1-3). The mouse IL-24 gene encodes for a 181 amino acid (aa) precursor that includes a 26 aa signal sequence and an 18 kDa (predicted), 155 aa mature segment (4). Unlike mature human IL-24, which contains three potential N-linked glycosylation sites, mouse mature IL-24 has only one. In human, both glycosylation and the presence of an intrachain disulfide bond have been found to be necessary for full activity (5). This requirement for glycosylation is unique among IL-10 family members. Presumably, the same structural modifications apply to mouse IL-24. Mouse IL-24 is a 27 kDa protein when secreted, and is active on human receptors (1, 3). Mature mouse IL-24 shares 69%, 84%, 69%, 68% and 61% aa sequence identity with human, rat, equine, canine and bovine IL-24, respectively. A 119 aa isoform termed FISP-sp diverges at aa 77. FISP-sp has been found mainly in the endoplasmic reticulum of Th2 cells. It can dimerize with intracellular IL-24, and antagonize the pro-apoptotic effects of IL-24 (6). Under physiological conditions, cytokine-stimulated monocytes/macrophages and Th2 cells produce most secreted IL-24; other sources include B cells, keratinocytes, NK cells, and differentiating melanoma cells (1-3, 7-9). Secreted IL-24 binds and signals through complexes of IL‑20 R alpha:IL-20 R beta, or IL-22 R:IL-20 R beta (10). The IL-20 R alpha:IL-20 R beta complex is also a receptor for IL-19 and IL-20, while IL-22 R:IL-20 R beta binds IL‑20 (2, 10).  Both receptors are widely expressed, but have not been found on hematopoietic cells (3). Even so, IL-24 induces type I proinflammatory cytokines in monocytes, and inhibits plasma cell differentiation in germinal center B cells (8, 11). The phenotype of mice transgenic for IL-24 is similar to that of IL-20 and IL-22 transgenic mice, indicating overlap in their activities, particularly in the skin (12). Secreted IL-24 is anti-angiogenic, binding receptors on endothelial cells and blocking their differentiation (13). Intratumoral injection of adenovirus coding for IL-24 causes tumor-specific apoptosis in humans, but it is not clear whether secreted IL-24 contributes to this effect (14).

References

  1. Schaefer, G. et al. (2001) J. Immunol. 166:5859.
  2. Trivella, D.B.B. et al. (2010) Cell. Mol. Life Sci. 67:2909.
  3. Wang, M. and P. Liang (2005) Immunology 114:166.
  4. Swissprot Accession # Q925J3.
  5. Fuson, K.L. et al. (2009) J. Biol. Chem. 284:30526.
  6. Sahoo, A. et al. (2008) J. Biol. Chem. 283:28860.
  7. Poindexter, N.J. et al. (2005) J. Leukoc. Biol. 78:745.
  8. Maarof, G. et al. (2010) Blood 115:1718.
  9. Poindexter, N.J. et al. (2010) Exp. Dermatol. 19:714.
  10. Wang, M. et al. (2002) J. Biol Chem. 227:7341.
  11. Caudell, E.G. et al. (2002) J. Immunol. 168:6041.
  12. He, M. and P. Liang (2010) J. Immunol. 184:1793.
  13. Ramesh, R. et al. (2003) Cancer Res. 63:5105.
  14. Emdad, L. et al. (2009) Cancer Biol. Ther. 8:391.

Long Name

Interleukin 24

Alternate Names

C49A, FISP, IL24, MDA-7, MOB-5, ST16

Entrez Gene IDs

11009 (Human); 93672 (Mouse); 170819 (Rat)

Gene Symbol

IL24

UniProt

NP_444325

Additional IL-24 Products

Product Documents for Recombinant Mouse IL-24 (NS0-expressed) Protein

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS 7807-ML (with carrier)
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Product Specific Notices for Recombinant Mouse IL-24 (NS0-expressed) Protein

For research use only

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