Recombinant Mouse IL-28 R alpha/IFN-lambda R1 Fc Chimera, CF

R&D Systems, part of Bio-Techne | Catalog # 5384-MR

R&D Systems, part of Bio-Techne
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5384-MR-050
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Key Product Details

Source

NS0

Accession #

AAH57856

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-28 R alpha/IFN-lambda R1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse IL-28 R alpha/IFN-lambda R1 protein
Mouse IL-28 R alpha
(Arg21-Ala227)
Accession # AAH57856		 IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg21

Predicted Molecular Mass

50.6 kDa (monomer)

SDS-PAGE

62-70 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
1 μg/mL of Recombinant Mouse IL-28 R alpha/IFN-lambda  R1 Fc Chimera was mixed with serially diluted Recombinant Mouse (rm) IL-28B/IFN-lambda 3 (Catalog # 1789-ML). Following incubation, the IL-28 R alpha rmIL-28B complex was captured in a goat anti-mFc-coated plate. Bound IL-28B was measured using biotinylated goat anti-mIL-28B. The concentration of rmIL-28B that produces 50% of the optimal binding was found to be approximately 0.7-3.5 μg/mL.

Formulation, Preparation and Storage

5384-MR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-28 R alpha/IFN-lambda R1

IL-28 R alpha (IL-28 receptor alpha subunit; also named interferon-lambda R1) is a type I transmembrane glycoprotein that is cytokine receptor family 2 member 12 (CRF2-12) (1-4). It pairs with the IL-10 receptor beta subunit (IL-10 R beta, CRF2-4) to form the IL-28 R (1-4). Each subunit of this receptor can interact with the interferon-like cytokines (type III interferons) IL-28A (IFN-lambda 2), IL-28B (IFN-lambda 3) and IL-29 (IFN-lambda 1). Mouse IL 28A and IL-28B share 97% amino acid (aa) identity; rodents do not express IL-29 (1-4). Mouse IL-28 R alpha cDNA encodes a 535 aa protein with a 20 aa signal peptide, a 207 aa extracellular domain (ECD) with a fibronectin type III motif and four potential N-glycosylation sites, a 21 aa transmembrane sequence, and a proline-rich and acidic 287 aa cytoplasmic domain. A 254 aa splice variant of mouse IL 28 R alpha diverges at aa 224 and lacks a transmembrane sequence (3, 5). The mature mouse IL-28 R alpha ECD shares 83%, 67%, 64% and 63% aa sequence identity with rat, human, canine and bovine IL-28 Ra, respectively. Mouse IL-28 R will respond to human IL-28A (6). IL-28 R is constitutively expressed in most tissues, but its ligands are mainly produced by antigen presenting cells in response to viruses and their products (2-6). Signaling through IL-28 R alpha is similar to that of receptors for type I IFNs and involve tyrosine phosphorylation, activation of JAK tyrosine kinases, STAT phosphorylation and formation of the IFN-stimulated regulatory factor 3 (ISGF-3) transcription factor complex (1-7). This signaling pathway induces antiviral activity and up-regulates MHC class I antigen expression (2-7). Anti-proliferative activity has also been shown for IL-28/IL-28 R (7).

References

  1. Chen, Q. et al. (2006) Vitam. Horm. 74:207.
  2. Meager, A. et al. (2005) Cytokine 31:109.
  3. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
  4. Kotenko, S.V. et al. (2003) Nat. Immunol. 4:69.
  5. Entrez Accession # EDL29971.
  6. Lasfar, A. et al. (2006) Cancer Res. 66:4468.
  7. Dumoutier, L. et al. (2004) J. Biol. Chem. 279:32269.

Long Name

Interleukin 28 Receptor alpha

Alternate Names

CRF2-12, IFN-lambda R1, IFNLR1, IL-28Ra, IL28R alpha, IL28RA, LICR2

Entrez Gene IDs

163702 (Human); 242700 (Mouse); 362628 (Rat)

Gene Symbol

IFNLR1

UniProt

AAH57856

Additional IL-28 R alpha/IFN-lambda R1 Products

Product Documents for Recombinant Mouse IL-28 R alpha/IFN-lambda R1 Fc Chimera, CF

Product Datasheets

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Product Specific Notices for Recombinant Mouse IL-28 R alpha/IFN-lambda R1 Fc Chimera, CF

For research use only

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