Recombinant Mouse IL-36 beta/IL-1F8 (aa 31-183) Protein

R&D Systems, part of Bio-Techne | Catalog # 7060-ML

R&D Systems, part of Bio-Techne
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Carrier Free
7060-ML-010/CF
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7060-ML-010
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Key Product Details

Source

E. coli

Accession #

Q9D6Z6

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-36 beta/IL-1F8 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived mouse IL-36 beta/IL-1F8 protein
Ser31-Lys183

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser31

Predicted Molecular Mass

17.4 kDa

SDS-PAGE

16 kDa, reducing conditions

Activity

Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
The ED50 for this effect is 1-6 ng/mL.

Scientific Data Images for Recombinant Mouse IL-36 beta/IL-1F8 (aa 31-183) Protein

Recombinant Mouse IL-36 beta/IL-1F8 (aa 31-183) Protein Bioactivity

Recombinant Mouse IL-36 beta/IL-1F8 (aa 31-183) Protein Bioactivity

Recombinant Mouse IL-36 beta/IL-1F8 (aa 31-183) (Catalog # 7060-ML) induces IL-6 secretion in the NIH‑3T3 mouse embryonic fibroblast cell line. The ED50 for this effect is 1-6 ng/mL.
Recombinant Mouse IL-36 beta/IL-1F8 (aa 31-183) Protein SDS-PAGE

Recombinant Mouse IL-36 beta/IL-1F8 (aa 31-183) Protein SDS-PAGE

1 µg/lane of Recombinant Mouse IL-36 beta/IL-1F8 (aa 31-183) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 16 kDa.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 7060-ML
Formulation Lyophilized from a 0.2 μm filtered solution in MES, NaCl, TCEP, EDTA, CHAPS and PEG 8000 with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in 10 mM Tris-HCl, pH 8.0.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 7060-ML/CF
Formulation Lyophilized from a 0.2 μm filtered solution in MES, NaCl, TCEP, EDTA, CHAPS and PEG 8000 with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in 10 mM Tris-HCl, pH 8.0.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-36 beta/IL-1F8

Mouse interleukin‑36 beta [IL-36 beta; previously IL‑1F8, FIL‑1 eta (eta) and IL‑1H2] is a member of the IL‑1 family of proteins that includes IL‑1 beta, IL‑1 alpha, IL‑1ra, IL‑18, IL-36Ra/IL-1F5, IL‑36 alpha/IL‑1F6, IL‑37/IL‑1F7, IL‑36 gamma/IL‑1F9 and IL‑1F10 (1 ‑ 6). All family members show a 12 beta‑stranded beta‑trefoil configuration, share up to 50% amino acid (aa) sequence identity, and are believed to have arisen from a common ancestral gene (3, 4). Although two alternatively spliced transcript variants for human IL‑36 beta/IL‑1F8 have been described, to date, only one mouse IL‑36 beta/IL‑1F8 isoform is known (3). Mouse IL‑36 beta/IL‑1F8 is synthesized as a 183 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N‑linked glycosylation site(s) (1, 2). Mouse IL‑36 beta/IL‑1F8 shares 61% and 74% aa identity with human IL‑36 beta isoform 2 and rat IL‑36 beta, respectively. IL‑36 beta is agonistic, stimulating release of inflammatory mediators such as IL‑6 and IL‑8, and cytotoxic peptides such as beta-defensins 2 and 3 that aid in defense against microbial pathogens (7 ‑ 10). The receptor for IL-36 proteins is IL‑1 Rrp2, with IL‑1 RAcP as a coreceptor (7, 9). Antagonism of IL‑36 proteins by IL‑36Ra, which also binds IL‑1 Rrp2, has been shown by some investigators (5, 6). Skin keratinocytes express highest levels of IL‑36 proteins and their receptors, followed by epithelia in the esophagus, trachea and bronchae (7 ‑ 9). IL‑36 beta expression is increased in psoriatic skin and may play a role in pathogenesis of psoriasis (7, 8). IL‑36 beta is also expressed in resting and activated monocytes and B cells, synovial fibroblasts, neurons and glia, and is detectable in plasma and body fluids (1, 7, 9, 11). IL‑36 beta, along with IL‑36 alpha and IL‑36 gamma, is up‑regulated by IL‑1 alpha and TNF‑ alpha in keratinocytes, and has been shown to activate NF‑ kappaB and MAPK signaling pathways in an IL‑1 Rrp2‑dependent manner (7 ‑ 9). Full‑length recombinant IL‑36 proteins appear less active than their endogenous counterparts, but trimming of the N‑termini enhances their activity (9, 12).

References

  1. Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
  2. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
  3. Nicklin, M.J.H. et al. (2002) Genomics 79:718.
  4. Dunn, E. et al. (2001) Trends Immunol. 22:533.
  5. Dinarello, C. et al. (2010) Nat. Immunol. 11:973.
  6. Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.
  7. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
  8. Johnston, A. et al. (2011) J. Immunol. 186:2613.
  9. Magne, D. et al. (2005) Arthritis Res. Ther. 8:R80.
  10. van Asseldonk, E.J.P. et al. (2010) Obesity 18:2234.
  11. Wang, P. et al. (2005) Cytokine 29:245.
  12. Blumberg, H. et al. (2010) J. Immunol. 185:4354.

Long Name

Interleukin 36 beta/Interleukin 1 Family 8

Alternate Names

FIL1 eta, IL-1 eta, IL-1H2, IL1F8, IL36 beta, IL36B

Entrez Gene IDs

27177 (Human); 69677 (Mouse)

Gene Symbol

IL36B

UniProt

Q9D6Z6

Additional IL-36 beta/IL-1F8 Products

Product Documents for Recombinant Mouse IL-36 beta/IL-1F8 (aa 31-183) Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse IL-36 beta/IL-1F8 (aa 31-183) Protein

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