Recombinant Mouse Kallikrein 1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7928-SE

R&D Systems, part of Bio-Techne
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Key Product Details

Source

NS0

Accession #

P15947

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Kallikrein 1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Kallikrein 1 protein
Mouse Kallikrein 5 precursor
(Val23-Arg67)
Accession # NP_081082 		 Mouse Kallikrein 1
(Ile25-Asp261)
Accession # P15947		 6-His tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val23 (Mouse Kallikrein 5 precursor)

Predicted Molecular Mass

32 kDa

SDS-PAGE

40-45 kDa, reducing conditions

Activity

Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC).
The specific activity is >15,000 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

7928-SE
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Kallikrein 1

The kallikreins are a family of trypsin‑like serine proteases, many of which are associated with a variety of cancers (1). Kallikrein 1 (KLK1) is also known as tissue kallikrein and urinary kallikrein. An important physiological function of KLK1 is the cleavage of kininogen to release a vasoactive kinin peptide, bradykinin (for rodent KLK1) or lysyl‑bradykinin (for human KLK1) (2, 3). Kinins regulate vasodilation, blood pressure reduction, smooth muscle relaxation and contraction, pain induction and inflammation. Recombinant mouse KLK1 was expressed with a human CD33 signal peptide and a mouse KLK5 pro‑peptide, followed by the mouse KLK1 catalytic domain (residues 25 to 261). The recombinant mouse KLK1 was purified as the latent pro‑form, which is readily activated by treatment with thermolysin.

References

  1. Avgeris, M. et al. (2012) Biol. Chem. 393:301.
  2. Hosoi, K. et al. (1994) J. Biochem. 115:137.
  3. Kato, H. et al. (1987) J. Biochem. 102:1389.

Alternate Names

KLK1

Entrez Gene IDs

3816 (Human); 16612 (Mouse)

Gene Symbol

KLK1

UniProt

P15947

Additional Kallikrein 1 Products

Product Documents for Recombinant Mouse Kallikrein 1 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Mouse Kallikrein 1 Protein, CF

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