Recombinant Mouse Kell Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1454-ZN
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse Kell protein
Phe50-Trp713, with an N-terminal 7-His tag
Phe50-Trp713, with an N-terminal 7-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
His
Predicted Molecular Mass
76 kDa
SDS-PAGE
106 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH (Catalog # ES005).
The specific activity is >0.2 pmol/min/µg, as measured under the described conditions.
The specific activity is >0.2 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
1454-ZN
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and ZnCl2. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Kell
Kell, a type II membrane glycoprotein, is linked through a single disulfide bond to XK, a putative membrane transporter. The two proteins constitute the Kell blood group antigens (1). Kell is a zinc endopeptidase of the neprilysin (NEP) family which also includes endothelin converting enzymes (ECE-1 and ECE-2), PEX, XCE, DINE and several NEP-like proteins (2). It has been shown to cleave big endothelin-3 (ET-3) at Trp21-Ile22, yielding ET-3, and to a much lesser extent, big ET-1 and big ET-2 at Trp21-Val22, yielding ET-1 and ET-2 (3). Several different molecular defects cause the Kell null phenotype, which has no obvious clinical outcome (4).
References
- Lee, S. et al. (2000) Transfus. Med. Rev. 14:93.
- Turner, A.J. et al. (2001) BioEssays. 23:261.
- Lee, S. et al. (1999) Blood 94:1440.
- Lee, S. et al. (2001) J. Biol. Chem. 276:27281.
Long Name
Zinc protease blood group antigen
Alternate Names
CD238, KEL
Gene Symbol
KEL
UniProt
Additional Kell Products
Product Documents for Recombinant Mouse Kell Protein, CF
Product Specific Notices for Recombinant Mouse Kell Protein, CF
For research use only
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