Recombinant Mouse LILRC1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9288-T4

R&D Systems, part of Bio-Techne
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9288-T4-050
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Key Product Details

Source

NS0

Accession #

AAY44811.1

Conjugate

Unconjugated

Applications

Bioactivity

View all LILRC1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse LILRC1 protein
Gln17-Asn248, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln17 inferred from enzymatic pyroglutamate treatment revealing Glu18

Predicted Molecular Mass

27 kDa

SDS-PAGE

36-50 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse LILRC1 (Catalog # 9288-T4) is coated at 2 µg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) binds with an ED50 = 60-360 ng/mL.

Scientific Data Images for Recombinant Mouse LILRC1 Protein, CF

Recombinant Mouse LILRC1 Protein Bioactivity

Recombinant Mouse LILRC1 Protein Bioactivity

When Recombinant Mouse LILRC1 (Catalog # 9288-T4) is coated at 2 µg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) binds with an ED50 = 60-360 ng/mL.

Formulation, Preparation and Storage

9288-T4
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LILRC1

The leukocyte immunoglobulin-like receptors (LILR) comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIRs) (1). Mouse LILRC1 (leukocyte immunoglobulin-like receptor C1), also known as LILRA5, ILT11, LIR-9, and CD85f, consists of a 227 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 10 aa cytoplasmic tail (2, 3). The ECD contains two Ig-like domains (4), and the transmembrane segment contains a positively charged aspartic acid residue which may mediate its association with the signaling molecule, FcR common gamma chain (3, 5). Within the ECD, mouse LILRC1/LILRA5 shares 56% and 85% aa sequence identity with human and rat LILRA5, respectively. LILRA5 is expressed by monocytes, macrophages, and neutrophils (2, 3, 5). It is found as an approximately 40 kDa molecule on the cell surface, while a soluble 25 kDa form can be released into the synovial fluid of rheumatoid arthritis patients (2, 5). Cross-linking of LILRA5 on monocytes induces the expression of pro-inflammatory cytokines (IL-1 beta, IL-6, TNF-alpha) as well as the anti-inflammatory IL-10 (2, 5). R&D Systems in-house testing indicates that LILRC1/LILRA5 binds to Angiopoietin-like 7, consistent with the demonstrated functional interactions between other members of these protein families (6).

References

  1. Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
  2. Borges, L. et al. (2003) Blood 101:1484.
  3. Hoelsbrekken, S.E. et al. (2005) Immunogenetics 57:479.
  4. Shiroishi, M. et al. (2006) J. Biol. Chem. 281:19536.
  5. Mitchell, A. et al. (2008) Eur. J. Immunol. 38:3459.
  6. Zheng, J. et al. (2012) Nature 485:656.

Long Name

Leukocyte Immunoglobulin-like Receptor C1

Alternate Names

LILRA5

Entrez Gene IDs

232801 (Mouse); 691533 (Rat)

Gene Symbol

LILRA5

UniProt

AAY44811.1

Additional LILRC1 Products

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Product Specific Notices for Recombinant Mouse LILRC1 Protein, CF

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