Recombinant Mouse Lipocalin-13 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8534-LC

R&D Systems, part of Bio-Techne
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8534-LC-050
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Key Product Details

Source

E. coli

Accession #

Q8K1H9

Conjugate

Unconjugated

Applications

Bioactivity

View all Lipocalin-13 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived mouse Lipocalin-13 protein
Ala19-Asp176

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala19

Predicted Molecular Mass

18 kDa

SDS-PAGE

18 kDa, reducing conditions

Activity

Measured by its ability to bind all-trans-retinoic acid. The concentration of all-trans-retinoic acid required to quench 50% of Trp fluorescence in Recombinant Mouse Lipocalin-13 is approximately 20-120 µM.

Formulation, Preparation and Storage

8534-LC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lipocalin-13

Recombinant Mouse Lipocalin-13, also known as Odorant-Binding Protein 2a, is a member of the Lipocalin family of small secreted proteins, which are involved in a variety of biological processes including chemical communication, cell proliferation and differentiation, metabolism, reproduction, immune responses, and cancer development (1). Members of this family share a highly-conserved tertiary structure with a characteristic beta-barrel at the center (1). This central cavity binds to small lipophilic molecules, including fatty acids, retinol, steroids, odorants, and pheromones, allowing Lipocalins to regulate the transportation, stability, release, and activity of these molecules (1). Mouse Lipocalin-13 is synthesized as a 176 amino acid (aa) precursor that contains a 19 aa signal sequence and a 157 aa mature region. It has been suggested that the Lipocalin-13 precursor undergoes proteolytic processing because two isoforms with molecular weights between 20 and 29 kDa have been identified on SDS-PAGE (2). Mature mouse Lipocalin-13 shares 69% and 36% aa sequence identity with the rat and human orthologs, respectively.</p><p /><p>Lipocalin-13 is expressed and secreted by multiple tissues in mice including liver, pancreas, and skeletal muscle (2). It has been shown to regulate glucose and lipid metabolism (2, 3). Lipocalin-13 deficiency is associated with obesity and is thought to contribute to hyperglycemia, insulin resistance, glucose intolerance and hepatic steatosis in obese mice (2, 3). Additionally, Lipocalin-13 has been shown to have anti-diabetogenic effects. It suppresses hepatic glucose production, uptake of circulating glucose by adipocytes, and induction of fatty acid oxidation by liver, possibly by stimulating CPT-1 alpha production (1-3)

References

  1. Zhou, Y. and L. Rui (2013) Vitam. Horm. 91:369.
  2. Cho, K.W. et al. (2011) Mol. Cell. Biol. 31:450.
  3. Sheng, L. et al. (2011) J. Biol. Chem. 286:38128.

Alternate Names

LCN13, Lipocalin13, Obp2a

Entrez Gene IDs

227627 (Mouse)

Gene Symbol

OBP2A

UniProt

Q8K1H9 (Mouse)

Additional Lipocalin-13 Products

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Product Specific Notices for Recombinant Mouse Lipocalin-13 Protein, CF

For research use only

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