Recombinant Mouse LRRC32/GARP Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6229-LR

R&D Systems, part of Bio-Techne
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6229-LR-050
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Key Product Details

Source

CHO

Accession #

NP_001106850

Conjugate

Unconjugated

Applications

Bioactivity

View all LRRC32/GARP Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse LRRC32/GARP protein
Ile18-Asn628, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile18

Predicted Molecular Mass

67.4 kDa

SDS-PAGE

70-80 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When recombinant mouse LRRC32/GARP is immobilized at 5 μg/mL, recombinant human Latent TGF-beta binds with an apparent Kd <25 nM.

Formulation, Preparation and Storage

6229-LR
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl, PEG 3350 and CHAPS.
Reconstitution
Reconstitute at 300 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 2 weeks, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRRC32/GARP

Leucine-rich repeat protein 32 (LRRC32), also known as GARP (glycoprotein A repetitions predominant), is an 80 kDa type I transmembrane glycoprotein (1). Mature mouse LRRC32 consists of a 608 amino acid (aa) extracellular domain (ECD) that contains 22 leucine-rich repeats, a 21 aa transmembrane segment, and a 14 aa cytoplasmic domain (2-4). Within the ECD, mouse LRRC32 shares 80 and 94% aa sequence identity with human and rat LRRC32, respectively. LRRC32 is widely expressed during embryogenesis and on adult platelets (4, 5). Among T cells, it is selectively expressed on activated FOXP3+ regulatory T cells (Treg) (6-10). LRRC32 expression promotes the acquisition of a Treg phenotype including reduced cellular proliferation, reduced cytokine secretion, and the capacity to suppress the proliferation of naïve T cells (6-8). LRRC32 binds directly to the TGF-beta latency associated peptide (LAP) and tethers latent TGF-beta on the surface of activated Treg cells (9, 10). The presentation of TGF-beta on Tregs contributes to their ability to suppress naïve T cell proliferation (11).

References

  1. Battaglia, M. and M.G. Roncarolo (2009) Eur. J. Immunol. 39:3296.
  2. Ollendorff, V. et al. (1994) Cell Growth Differ. 5:213.
  3. Bella, J. et al. (2008) Cell Mol Life Sci. 65:2307.
  4. Roubin, R. et al. (1996) Int. J. Dev. Biol. 40:545.
  5. Macaulay, I.C. et al. (2007) Blood 109:3260.
  6. Wang, R. et al. (2008) PloS ONE 3:e2705.
  7. Wang, R. et al. (2009) Proc. Natl. Acad. Sci. 106:13439.
  8. Probst-Kepper, M. et al. (2009) J. Cell. Mol. Med. 13:3343.
  9. Tran, D.Q. et al. (2009) Proc. Natl. Acad. Sci. 106:13445.
  10. Stockis, J. et al. (2009) Eur. J. Immunol. 39:3315.
  11. Vignali, D.A. et al. (2008) Nat. Rev. Immunol. 8:523.

Long Name

Leucine-rich Repeat Containing 32/Glycoprotein A Repetitions Predominant

Alternate Names

D11S833E, GARP, Garpin

Entrez Gene IDs

2615 (Human); 434215 (Mouse)

Gene Symbol

LRRC32

UniProt

NP_001106850

Additional LRRC32/GARP Products

Product Documents for Recombinant Mouse LRRC32/GARP Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse LRRC32/GARP Protein, CF

For research use only

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