Recombinant Mouse Marapsin/Pancreasin Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1989-SE
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse Marapsin/Pancreasin protein
Ala23-Thr290, with a C-terminal 6-His tag
Ala23-Thr290, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala23 & Met38
Predicted Molecular Mass
28 kDa
SDS-PAGE
33 kDa, reducing conditions
Activity
Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Gly-Arg-ThioBenzyl ester (Z-GR-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.
The specific activity is >1,200 pmol/min/µg, as measured under the described conditions.
The specific activity is >1,200 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
1989-SE
| Formulation | Lyophilized from a 0.2 μm filtered solution in MES and NaCl. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 25 mM MES and 100 mM NaCl, pH 6.5. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Marapsin/Pancreasin
Marapsin, Pancreasin, and channel-activating protease 2 (CAP-2), encoded by the Prss27 gene, are different names given for the same serine protease that is expressed strongly in the pancreas (1). The mouse protein is synthesized with a signal peptide (amino acid residues 1‑22), a pro peptide (residues 23‑37) and a mature chain (residues 38‑290) corresponding to the serine protease domain. The full-length protein was expressed and the secreted protein purified. The N-terminal sequencing results indicate that the purified protein is a disulfide bond-linked dimer formed between the pro peptide and the mature chain. The active enzyme has low activity against peptide substrates tested, but high activity against thioester substrates. The peptidase activity is inhibited by 20 mM benzamidine.
References
- Bhagwandin, V.J. et al. (2003) J. Biol. Chem. 278:3363.
Alternate Names
CAP-2, MPN, Pancreasin, PRSS27
Gene Symbol
PRSS27
UniProt
Additional Marapsin/Pancreasin Products
Product Documents for Recombinant Mouse Marapsin/Pancreasin Protein, CF
Product Specific Notices for Recombinant Mouse Marapsin/Pancreasin Protein, CF
For research use only
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