Recombinant Mouse Matrilin-2 Protein
R&D Systems, part of Bio-Techne | Catalog # 3234-MN
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse Matrilin-2 protein
Arg24-Arg956 & Met40-Arg956, both with a C-terminal 6-His tag
Arg24-Arg956 & Met40-Arg956, both with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Arg24 & Met40
Predicted Molecular Mass
104.9 kDa
SDS-PAGE
120 kDa, reducing conditions
Activity
Measured by its binding ability in a functional ELISA.
Immobilized Collagen I at 10 µg/mL (100 µL/well) will bind Recombinant Mouse Matrilin‑2 with a linear range of 8-1,000 ng/mL.
Immobilized Collagen I at 10 µg/mL (100 µL/well) will bind Recombinant Mouse Matrilin‑2 with a linear range of 8-1,000 ng/mL.
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 3234-MN
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 3234-MN/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Matrilin-2
Matrilin-2 is an extracellular matrix protein that belongs to the superfamily of von Willebrand factor A domain (VWA) containing proteins. It is expressed in many tissues and functions as a bridging component between other matrix molecules (1, 2, 3, 4). The mouse Matrilin-2 cDNA encodes a 956 amino acid (aa) precursor with a 23 aa signal sequence, two VWA domains separated by ten tandem EGF-like repeats, and a C-terminal coiled coil domain (5). Mouse Matrilin-2 shares 84%-87% aa sequence identity with human, rat, and canine Matrilin-2, and 26%, 21%, and 34% aa sequence identity with mouse Matrilin-1, -3, and -4, respectively. Matrilin-2 forms a variety of disulfide-linked oligomers via its coiled coil domain (4, 6-8). It can assemble into homotrimers or heterotrimers with Matrilin-1 and/or Matrilin-4 (4, 6, 7) but has not been detected in heterotrimers containing Matrilin-3 (7). The VWA domains are thought to mediate Matrilin-Matrilin interactions as well as interactions with other matrix proteins such as Fibronectin, Collagen I, Fibrilin-2, and Laminin-1/Nidogen-1 complexes (6). Matrilin-2 knockout mice do not display any obvious abnormalities, suggesting that the expression of other molecules can compensate for the lack of Matrilin-2 (9).
References
- Wagener, R. et al. (2005) FEBS Lett. 579:3323.
- Deak, F. et al. (1999) Matrix Biol. 18:55.
- Whittaker, C.A. and R.O. Hynes (2002) Mol. Biol. Cell 13:3369.
- Piecha, D. et al. (1999) J. Biol. Chem. 274:13353.
- Deak, F. et al. (1997) J. Biol. Chem. 272:9268.
- Piecha, D. et al. (2002) Biochem. J. 367:715.
- Frank, S. et al. (2002) J. Biol. Chem. 277:19071.
- Pan, O.H. and K. Beck (1998) J. Biol. Chem. 273:14205.
- Mates, L. et al. (2004) Matrix Biol. 23:195.
Alternate Names
MATN2, Matrilin2
Entrez Gene IDs
4147 (Human)
Gene Symbol
MATN2
UniProt
Additional Matrilin-2 Products
Product Documents for Recombinant Mouse Matrilin-2 Protein
Product Specific Notices for Recombinant Mouse Matrilin-2 Protein
For research use only
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