Recombinant Mouse Mer Fc (HEK293-expressed) Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11314-MR

R&D Systems, part of Bio-Techne
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11314-MR-050
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Key Product Details

Source

HEK293

Accession #

Q60805.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Mer Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived mouse Mer protein
Mouse Mer
(Glu23-Phe498)
Accession # Q60805.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu23

Predicted Molecular Mass

79 kDa

SDS-PAGE

114-131 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Mer Fc Chimera (HEK293-expressed) (Catalog # 11314-MR) is immobilized at 2.00 µg/mL (100 µL/well), Recombinant Mouse Gas6 (Full Length) Protein  (Catalog # 8310-GS) binds with an ED50 of 0.400-3.60 µg/mL

Scientific Data Images for Recombinant Mouse Mer Fc (HEK293-expressed) Protein, CF

Recombinant Mouse Mer Fc Chimera (HEK293-expressed) Protein Binding Activity.

Measured by its binding ability in a functional ELISA. When Recombinant Mouse Mer Fc Chimera (HEK293-expressed) (Catalog # 11314-MR) is immobilized at 2.00 µg/mL (100 µL/well), Recombinant Mouse Gas6 (Full Length) Protein (8310-GS) binds with an ED50 of 0.400 -3.60 µg/mL

Recombinant Mouse Mer Fc (HEK293-expressed) Protein SDS-PAGE.

2 μg/lane of Recombinant Mouse Mer Fc (HEK293-expressed) Protein (Catalog # 11314-MR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 114-131 kDa and 228-262 kDa, respectively.

Formulation, Preparation and Storage

11314-MR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mer

Tyrosine-protein Kinase Mer, also known as c-Mer and MerTK, is a member of the receptor tyrosine kinase subfamily TAM (Tyro3, Axl, and Mer). Mature mouse Mer consists of 478 aa extracellular domain, 21 aa transmembrane domain, and 475 aa cytoplasmic domain. Within the extracellular domain, mouse Mer shares 77.1% and 90.4% homology with human and rat Mer, respectively. Similar to Axl and Tyro3, the extracelluar domain of Mer contains two Ig-like motifs and two fibronectin type III motifs. Mer is not expressed in normal B- and T-cells but expressed in neoplastic B- and T-cell lines (1-2).  It is also show higher expression in immunosuppressive M2‑like macrophages (3).  Mer is known to bind Gas6, Protein S, Tubby, Tubby-like protein 1 (Tulp1), and Galectin-3 (4-7). Upon binding ligands via the Ig-like motif, Mer is dimerized to trans-autophosphorylate the kinase domain to induce downstream signaling. It has been shown that Mer signaling in macrophages induces M2 polarization, which promote tumor growth, metastasis and evasion of anti-tumor immunity in tumor microenviroment (8). Inhibition of Mer, especially on leukocytes and macrophages, is an effective anti-cancer therapy (9).

References

  1. Graham, D.K. et al. (1994) Cell Growth Differ. 5:647.
  2. Graham, D.K. et al. (2006) Clin. Cancer Res. 12:2662.
  3. Shibata, T. et al. (2014) J. Immunol. 192:3569.
  4. Nagata, K. et al. (1996) J. Biol. Chem. 271:30022.
  5. Uehara, H. et al. (2008) J. Immunol. 180:2522.
  6. Caberoy, N.B. et al. (2010) EMBO J. 29:3898.
  7. Caberoy, N.B. et al. (2012) J. Cell Physiol. 227:401.
  8. Kim, S.Y. et al. (2016) Sci. Rep. 6:29673.
  9. Cummings, C.T. et al. (2013) Clin. Cancer Res. 19:5275.

Long Name

Receptor Tyrosine Protein Kinase Mer

Alternate Names

c-Eyk, C-mer, MerTK

Entrez Gene IDs

10461 (Human); 17289 (Mouse); 102143798 (Cynomolgus Monkey)

Gene Symbol

MERTK

UniProt

Q60805.1

Additional Mer Products

Product Documents for Recombinant Mouse Mer Fc (HEK293-expressed) Protein, CF

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Product Specific Notices for Recombinant Mouse Mer Fc (HEK293-expressed) Protein, CF

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