Recombinant Mouse MuSK Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10599-MK

R&D Systems, part of Bio-Techne
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Key Product Details

Source

NS0

Accession #

Q61006.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all MuSK Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse MuSK protein
Mouse MuSK
(Glu22-Thr494)
Accession # Q61006.1		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu22

Predicted Molecular Mass

79 kDa

SDS-PAGE

175-200, under non-reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
In the presence of Recombinant Mouse Agrin, immobilized Recombinant Mouse MuSK Fc Chimera (2 µg/mL at 100 µL/well) binds Recombinant Mouse LRP-4 (Catalog # 10229-LR) with an ED50 of 150-900 ng/mL

Scientific Data Images for Recombinant Mouse MuSK Fc Chimera Protein, CF

Recombinant MuSK Fc Chimera Protein Binding Activity.

In the presence of Recombinant Mouse Agrin, immobilized Recombinant Mouse MuSK Fc Chimera (2 μg/mL at 100 μL/well) binds Recombinant Mouse LRP-4 (10229-LR) with an ED50 of 150-900 ng/mL.

Formulation, Preparation and Storage

10599-MK
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 2 weeks, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MuSK

MuSK (muscle-specific kinase) is a 100-kDa type I transmembrane (TM) protein belonging to the receptor tyrosine kinase family (1). It is found in the postsynaptic membrane of skeletal muscle motor endplates (2). MuSK contains a 473 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 353 cytoplasmic domain. Human MuSK has multiple isoforms. One contains deletions at residues 307-394 and 454-461, while a second is a short soluble form that contains residues 120­-209 plus a unique 24 aa C­terminal tail (3). Within the ECD, mouse MuSK shares 91% and 96% aa sequence identity with human and rat MuSK, respectively. MuSK is a crucial signaling molecule in the formation of the neuromuscular junction(NMJ). Defects in MuSK causes muscle weakness in congenital myathenic syndromes (4). MuSK binds the heparin sulfate proteoglycan agrin to promote acetylcholine receptor clustering. It has also been found to bind with low-density lipoprotein receptor-related protein 4 (LRP4) (5), Wnt ligands (6), Biglycan (7), ColQ (8) and BMPs(10). Recent studies have showed that MuSK does not bind agrin directly, but enhanced the MuSK-LRP4 interaction (8, 9). When agrin binds to the N-terminal region of LRP4, this promotes the association of LRP4 and MuSK, which then stimulates MuSK kinase activity (11, 12).

References

  1. Valenzuela, D.M. et al. (1995) Neuron 15:573.
  2. Finn, A.J. et al. (2003) Nat. Neurosci. 6:717.
  3. Nasrin, F. et al. (2014) Sci. Rep. 4:6841.
  4. DeChiara, T. et al. (1996) Cell 85:501.
  5. Kim, N. et al. (2008) Cell 135:334.
  6. Banerjee, S. et al. (2011) Development 138:3287.
  7. Amenta, A.R. et al. (2012) J. Neurosci. 32:2324.
  8. Otsuka, K. et al. (2015) Scientific Reports 5:13928.
  9. Zhang, B. et al. (2008) Neuron 60:285.
  10. Yilmaz, A. et al. (2016) Science Signaling 9:ra87.
  11. Zhang, W. et al. (2011) J. Biol. Chem. 286:40624.
  12. Burden, S.J. et al. (2017) CSH Perspectives Biol. 5:a009167.

Long Name

Muscle-specific Receptor Tyrosine Kinase

Alternate Names

EC 2.7.10, EC 2.7.10.1, MGC126323, MGC126324, muscle, skeletal, receptor tyrosine kinase, MuSK, skeletal receptor tyrosine-protein kinase

Entrez Gene IDs

4593 (Human); 18198 (Mouse); 81725 (Rat); 102127677 (Cynomolgus Monkey)

Gene Symbol

MUSK

UniProt

Q61006.1

Additional MuSK Products

Product Documents for Recombinant Mouse MuSK Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse MuSK Fc Chimera Protein, CF

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