Recombinant Mouse Neuroglycan C/CSPG5 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5665-NG

R&D Systems, part of Bio-Techne
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5665-NG-050
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Key Product Details

Source

CHO

Accession #

AAH55736

Conjugate

Unconjugated

Applications

Bioactivity

View all Neuroglycan C/CSPG5 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Neuroglycan C/CSPG5 protein
Val31-Gln420, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val31

Predicted Molecular Mass

42.1 kDa

SDS-PAGE

85-105 kDa, reducing conditions

Activity

Measured by its ability to inhibit Recombinant Human Midkine (Catalog # 258-MD) induced neurite outgrowth in E16-E18 rat embryonic cortical neurons.
When 5 x 104 neurons per well are added to a plate pre-incubated with serial dilutions of rmNeuroglycan-C and 1 μg/mL of rhMidkine, the neurite outgrowth promotion is significantly inhibited in a dose dependent manner with an effect at
0.6-1.2 μg/mL.

Formulation, Preparation and Storage

5665-NG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuroglycan C/CSPG5

Neuroglycan C (NGC; also CSPG5 and CALEB) is a 120 - 150 kDa type I transmembrane glycoprotein and member of the neuregulin family of proteins (1 - 2). Depending on its expression profile, NGC may be a glycoprotein of 120 kDa, or a chondroitin sulfate (CS) proteoglycan of 150 kDa (2 - 3). Mouse NGC is synthesized as a 566 amino acid (aa) precursor that contains a 30 aa signal sequence, a 393 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 122 aa cytoplasmic region. The ECD contains one CS attachment domain (aa 32 - 273), with CS attachment at Ser117, one EGF-like domain (aa 371 - 413), three potential sites for N-linked glycosylation, and ten potential sites for O-linked glycosylation (4). Splicing variants produce four isoforms for human NGC. Isoform 1 is the standard form. Isoform 2 has a deletion of aa 487 - 513, while isoform 3 has an alternative start site at Met82 and the same deletion. Isoform 4 has a 56 aa substitution for aa 514 - 566. Phosphorylation likely occurs at Ser249, and proteolysis generates a 75 kDa soluble fragment (5). Over aa 31 - 420, mouse NGC shares 84% aa identity with human NGC. NGC is expressed in nervous tissue and is found on retinal ganglion cells, cerebellar Purkinje cells and hippocampal neurons (6). NGC may function as a growth and differentiation factor involved in neuritogenesis. One study shows that the recombinant ectodomain of NGC core protein enhances neurite outgrowth from rat neocortical neurons in culture via phosphatidylinositol 3-kinase and protein kinase C signaling pathways (7). Another study states that NGC is a novel component of midkine receptors, a heparin-binding growth factor that promotes cell attachment and process extension in oligodendroglial precursor-like cells (3). NGC also acts as a growth factor by directly binding ERbB3 tyrosine kinase and transactivating ErbB2 (1).

References

  1. Kinugasa, Y. et al. (2004) Biochem. Biophys Res. Commun 321:1045.
  2. Yasuda, Y. et al. (1998) Neurosci. Res. 32:313.
  3. Ichihara-Tanaka, K. et al. (2006) J. Biol. Chem. 281:30857.
  4. Aono, S. et al. (2004) J. Biol. Chem. 279:46536.
  5. Shuo, T. et al. (2007) J. Neurochem. 102:1561.
  6. Aono, S. et al. (2006) J. Neurosci. Res.83:110.
  7. Nakanishi, K., et al. (2006) J. Biol. Chem. 281:24970.

Alternate Names

CALEB, CSPG5, NGC

Entrez Gene IDs

10675 (Human); 29873 (Mouse); 50568 (Rat)

Gene Symbol

CSPG5

UniProt

AAH55736 (Human)

Additional Neuroglycan C/CSPG5 Products

Product Documents for Recombinant Mouse Neuroglycan C/CSPG5 Protein, CF

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Product Specific Notices for Recombinant Mouse Neuroglycan C/CSPG5 Protein, CF

For research use only

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