Recombinant Mouse NPDC-1 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10970-NP

R&D Systems, part of Bio-Techne
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10970-NP-050
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Key Product Details

Source

NS0

Accession #

Q64322.2

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all NPDC-1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse NPDC-1 protein
Mouse NPDC-1
(Ala32-Thr190, Thr33Leu)
Accession # Q64322.2		 IEGRMD Human IgG1 Fc
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala32 & Arg35

Predicted Molecular Mass

43 kDa

SDS-PAGE

54-62 kDa, under reducing conditions.

Activity

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Recombinant Mouse NPDC-1 Fc Chimera, immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly enhance neurite outgrowth.

Scientific Data Images for Recombinant Mouse NPDC-1 Fc Chimera Protein, CF

Recombinant Mouse NPDC-1 Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Mouse NPDC-1 Fc Chimera Protein (Catalog # 10970-NP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 54-62 kDa and 108-124 kDa, respectively.

Formulation, Preparation and Storage

10970-NP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NPDC-1

Neural proliferation and differentiation control protein 1 (NPDC1) is an O-linked glycoprotein primarily expressed in lung and neural tissues and is involved in the regulation of cellular proliferation and differentiation (1,2). Mature NPDC1 consists of an extracellular domain (ECD) with a potential HLH-like domain, a transmembrane domain and a cytoplasmic domain containing a PEST motif (rich in proline, glutamine, serine, and threonine) which targets the protein for degradation (1). The ECD of mouse NPDC1 shares 76% and 92% amino acid sequence identity with human and rat NPDC1, respectively. NPDC1 expression is developmentally regulated and persists in the adult. NPDC1 binds a variety of cyclins and regulates differentiation events in neuronal precursor cells. It is expressed when neuronal precursor cells stop to divide and begin to differentiate (3). NPDC1 can interact with the transcription factor E2F-1, which results in the inhibition of E2F-1 binding to DNA and the corresponding transcription events (4). NPDC1 has been identified as a novel ligand for PILR alpha, binding through sialylation-dependent recognition (5).

References

  1. Spencer, M.L. et al. (2004) J. Biol. Chem. 279:37069.
  2. Evrard, C. and P. Rouget (2005) J. Neurosci. Res. 79:747.
  3. Galiana, E. et al. (1995) Proc. Natl. Acad. Sci. U.S.A. 92:1560.
  4. Sansal, I. et al. (2000) Oncogene 19:5000.
  5. Sun, Y. et al. (2012) J Biol Chem. 287:15837.

Long Name

Neural Proliferation, Differentiation and Control, 1

Alternate Names

CAB1, NPDC1

Entrez Gene IDs

56654 (Human); 18146 (Mouse); 296562 (Rat)

Gene Symbol

NPDC1

UniProt

Q64322.2

Additional NPDC-1 Products

Product Documents for Recombinant Mouse NPDC-1 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse NPDC-1 Fc Chimera Protein, CF

For research use only

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