Recombinant Mouse Osteocrin Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9700-ON

R&D Systems, part of Bio-Techne
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9700-ON-050
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Key Product Details

Source

E. coli

Accession #

P61364

Conjugate

Unconjugated

Applications

Bioactivity

View all Osteocrin Proteins and Enzymes »

Product Specifications

Source

E. coli-derived mouse Osteocrin protein
Val28-Gly130, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val28

Predicted Molecular Mass

12 kDa

SDS-PAGE

14-18 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse NPRC/NPR3 Fc Chimera (Catalog # 10187-NR) is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse Osteocrin that produces 50% of the optimal binding response is 0.6-3.6 ng/mL.

Scientific Data Images for Recombinant Mouse Osteocrin Protein, CF

Recombinant Mouse Osteocrin Protein Binding Activity

Recombinant Mouse Osteocrin Protein Binding Activity

When Recombinant Mouse NPRC/NPR-3 Fc Chimera (Catalog # 10187-NR) is immobilized at 2 µg/mL, Recombinant Mouse Osteocrin (Catalog # 9700-ON) binds with an ED50 of 0.6-3.6 ng/mL.

Formulation, Preparation and Storage

9700-ON
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Osteocrin

Osteocrin, also known as Musclin, is a secreted protein that is primarily expressed in bone and muscle. It is synthesized as a proprotein (11 kDa) that undergoes proteolytic processing to generate a mature 50 amino acid C-terminal active peptide (1). It was found to modulate osteoblast differentation and to regulate glucose metabolism in muscles (2). Mouse Osteocrin proprotein shares 77% and 78% amino acid sequence identity with the rat and human protein, respectively. Secretion of Osteocrin has been shown to be increased with exercise and is associated with metabolically beneficial formation of brown fat (3). Osteocrin has also been attributed to increases in exercise endurance through promotion of mitochondrial biosynthesis (4). Based on similarities with NPs (natriuretic peptides), Osteocrin was found to interact with NP clearance receptor NPR-C. This interaction is thought to modulate the availability of NPs, importantly increasing CNP which has been shown to stimulate endochondral ossification and elongate bones (5). In primates, Osteocrin may have evolved to regulate neuronal structure and function (6).

References

  1. Nishizawa, H. et al. (2004) Journal of Biological Chemistry, 279:19391.
  2. Thomas, G. et al. (2003) Journal of Biological Chemistry, 278:50563.
  3. Jeramic N. et al. (2017) J Cell Physiol 232:61.
  4. Subbatino, E. et al. (2015) Proc. Acad. Natl. Sci. U. S. A. 112:16042.
  5. Kanai, Y. et al. (2017) J of Clin Invest 127: 4136
  6. Ataman, B. et al. (2016) Nature 539:242.

Alternate Names

Musclin, OSTN

Entrez Gene IDs

344901 (Human); 239790 (Mouse)

Gene Symbol

OSTN

UniProt

P61364

Additional Osteocrin Products

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