Recombinant Mouse Plexin B3 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10220-PC

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
10220-PC-050
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

CHO

Accession #

Q9QY40.2

Conjugate

Unconjugated

Applications

Bioactivity

View all Plexin B3 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Plexin B3 protein
His35-Gln1245, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His35 & Gln140

Predicted Molecular Mass

131 kDa

SDS-PAGE

150-170 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Plexin B3 His tag (Catalog # 10220-PC) is immobilized at 2 μg/mL, 100 μL/well, Recombinant Mouse Semaphorin 4D/CD100 Fc Chimera  (Catalog # 5235-S4B) binds with an ED50 of 0.8-4.8 μg/mL.

Scientific Data Images for Recombinant Mouse Plexin B3 His-tag Protein, CF

2 μg/lane of Recombinant Mouse Plexin B3 His-tag Protein (Catalog # 10220-PC) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 150-170 kDa.

Formulation, Preparation and Storage

10220-PC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Plexin B3

Plexin B3 is a type I transmembrane glycoprotein that belongs to the Plexin family of semaphorin receptors which are critical in axon guidance, (1, 2). There are nine mammalian plexins divided into 4 classes all sharing a common architecture: An extracellular domain (ECD) that binds the semaphorin ligand, a single transmembrane domain and a conserved ~600 amino acid (aa) cytoplasmic region (1, 2). The ECD of mouse Plexin B3 consists of a Sema domain, three plexin-semaphorin-integrin (PSI) domains and four Ig-like, plexins, transcription factors (IPT) domains and shares 81% and 95% aa identity with human and rat Plexin B3, respectively. Alternative splicing of Plexin B3 results in at least three different isoforms (3). Plexin B3 is predominantly expressed in the brain and is a highly potent stimulator of neurite outgrowth (3). Plexin B3 and its identified ligand, the transmembrane semaphorin Sema5A, are both expressed during differentiation and migration of central nervous system oligodendrocytes (4 - 6). Plexin B3 is also expressed in peripheral cells such as fibroblasts, epithelial and primary endothelial cells. Plexin B3 activation by sema 5A mediates functional responses in these cells (4). Expression of Sema 5A and plexin B3 in gastric carcinoma contributes to invasion and metastasis of gastric carcinoma (7).

References

  1. Negishi, M. et al. (2005) Cell. Mol. Life Sci. 62:1363.
  2. Tamagnone, L. et al. (1999) Cell 99:71.
  3. Hartwig, C. et al. (2005) BMC Neurosci. 6:53.
  4. Artigiani, S. et al. (2004) EMBO Rep. 5:710.
  5. Goldberg, J.L. et al. (2004) J. Neurosci. 24:4989.
  6. Worzfeld, T. et al. (2004) Eur. J. Neurosci. 19:2622.
  7. P.G. et al. (2009) World. J. Gastroenterol. 15(22):2800.

Alternate Names

PLEXB3, PLEXR, PLXN6, PLXNB3

Entrez Gene IDs

5365 (Human); 140571 (Mouse); 363517 (Rat)

Gene Symbol

PLXNB3

UniProt

Q9QY40.2

Additional Plexin B3 Products

Product Documents for Recombinant Mouse Plexin B3 His-tag Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Mouse Plexin B3 His-tag Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.