Recombinant Mouse QSOX1/Quiescin Q6 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9257-QS

R&D Systems, part of Bio-Techne
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9257-QS-020
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Key Product Details

Source

E. coli

Accession #

Q8BND5

Conjugate

Unconjugated

Applications

Enzyme Activity

View all QSOX1/Quiescin Q6 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived mouse QSOX1/Quiescin Q6 protein
Ser36-Ala550
with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

59 kDa

SDS-PAGE

56 kDa, reducing conditions

Activity

Measured by its ability to produce hydrogen peroxide during the oxidation of Dithiothreitol (DTT).
The specific activity is >1,200 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

9257-QS
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: QSOX1/Quiescin Q6

Sulfhydryl Oxidase-1 (QSOX1) is an enzyme that contains thioredoxin and sulfhydryl oxidase domains (1-3). It is synthesized with a C-terminal transmembrane segment, but soluble secreted forms can be generated by alternative splicing or proteolytic shedding within the Golgi (4). Within the region encompassing both enzymatic domains and the central region, mouse QSOX1 shares 79% and 92% aa sequence identity with human and rat QSOX1, respectively. It plays a role in nascent protein folding by mediating disulfide oxidation (4-6). This activity is required for Laminin incorporation into the extracellular matrix (7). QSOX1 is up-regulated in many cancers and supports tumor cell proliferation and invasion (1, 8).

References

  1. Lake, D.F. and D.O. Faigel (2014) Antioxid. Redox. Signal. 21:485.
  2. Kodali, V.K. and C. Thorpe (2010) Antioxid. Redox Signal. 13:1217.
  3. Matsuba, S. et al. (2002) J. Dermatol. Sci. 30:50.
  4. Rudolf, J. et al. (2013) Biochem. J. 454:181.
  5. Heckler, E.J. et al. (2008) Biochemistry 47:4955.
  6. Alon, A. et al. (2012) Nature 488:414.
  7. Ilani, T. et al. (2013) Science 341:74.
  8. Katchman, B.A. et al. (2011) Mol. Cancer Res. 9:1621.

Long Name

Quiescin Q6 Sulfhydryl Oxidase 1

Alternate Names

HQSOX, QSCN6, Quiescin Q6, Sulfhydryl Oxidase-1

Entrez Gene IDs

5768 (Human); 104009 (Mouse); 84491 (Rat)

Gene Symbol

QSOX1

UniProt

Q8BND5

Additional QSOX1/Quiescin Q6 Products

Product Documents for Recombinant Mouse QSOX1/Quiescin Q6 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse QSOX1/Quiescin Q6 Protein, CF

For research use only

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