Recombinant Mouse Reg3G Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8189-RG

R&D Systems, part of Bio-Techne
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8189-RG-050
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Key Product Details

Source

E. coli

Accession #

O09049

Conjugate

Unconjugated

Applications

Bioactivity

View all Reg3G Proteins and Enzymes »

Product Specifications

Source

E. coli-derived mouse Reg3G protein
Glu27-Ala174, with an N-terminal Met

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met-Glu27

Predicted Molecular Mass

16 kDa

SDS-PAGE

16 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using RT4-D6P2T rat schwannoma cells.
The ED50 for this effect is 0.1-0.5 μg/mL.

Scientific Data Images for Recombinant Mouse Reg3G Protein, CF

Recombinant Mouse Reg3G Protein Binding Activity

Recombinant Mouse Reg3G Protein Binding Activity

Recombinant Mouse Reg3G (Catalog # 8189-RG) stimulates cell proliferation of RT4‑D6P2T rat schwannoma cells. The ED50 for this effect is 0.1-0.5 μg/mL.

Formulation, Preparation and Storage

8189-RG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Reg3G

Regenerating islet-derived protein 3-gamma (Reg3G), also known as pancreatitis-associated protein 3 (PAP3), is one of several secreted Reg proteins that contain a single C-type lectin domain and contribute to anti-microbial immunity in the gut (1, 2). Reg3G is secreted as a 148 amino acid (aa) proprotein, and 11 residues are cleaved from the N-terminus to generate the 15 kDa mature protein (3). This short propeptide serves as an inhibitor that limits the activity of the proprotein (3, 4). Mature mouse Reg3G shares 66% and 88% aa sequence identity with human and rat Reg3G, respectively. Reg3G is expressed by Paneth cells of the small intestine and is released into the gut lumen (5). It is up-regulated in gastrointestinal epithelium by bacterial inflammation or by defects in the mucous lining (5-8). Its expression and secretion is also induced in the lung in response to MRSA (drug resistant S. aureus) and in damaged skeletal muscle and peripheral neurons (4, 9). Reg3G binds to bacterial peptidoglycans, exerts bacteriostatic and bactericidal activities, and protects the mucosa against bacterial invasion (4, 5, 10, 11). It also inhibits inflammation by limiting the number of Th1 cells in the lamina propria (11). R&D in-house data indicate that Reg3G can induce the proliferation of Schwann cells, a property it shares with Reg3B (12).

References

  1. Parikh, A. et al. (2012) Biomol. Concepts 3:57.
  2. Narushima, Y. et al. (1997) Gene 185:159.
  3. Mukherjee, S. et al. (2009) J. Biol. Chem. 284:4881.
  4. Choi, S-M. et al. (2013) J. Exp. Med. 210:551.
  5. Cash, H.L. et al. (2006) Science 313:1126.
  6. Lee, K.S. et al. (2012) PLoS ONE 7:e30786.
  7. Keilbaugh, S.A. et al. (2005) Gut 54:623.
  8. Burger-van Passen, N. et al. (2012) PLoS ONE 7:e38798.
  9. Klasan, G.S. et al. (2014) Muscle Nerve 49:61.
  10. Brandl, K. et al. (2007) J. Exp. Med. 204:1891.
  11. Vaishnava, S. et al. (2011) Science 334:255.
  12. Livesey, F.J. et al. (1997) Nature 390:614.

Long Name

Regenerating Islet-derived III gamma

Alternate Names

LPPM429, PAP1B, PAP3, PAPIB, PAPIII, UNQ429

Entrez Gene IDs

130120 (Human); 19695 (Mouse); 24620 (Rat)

Gene Symbol

REG3G

UniProt

O09049

Additional Reg3G Products

Product Documents for Recombinant Mouse Reg3G Protein, CF

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Product Specific Notices for Recombinant Mouse Reg3G Protein, CF

For research use only

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