Recombinant Mouse Renin Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 4277-AS

R&D Systems, part of Bio-Techne
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4277-AS-020
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Key Product Details

Source

NS0

Accession #

P06281

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Renin Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Renin protein
Leu22-Arg402, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu22

Predicted Molecular Mass

43 kDa

SDS-PAGE

45-55 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate 5-FAM/QXL™ 520. Peti-Peterdi, J. et al. (2009) Physiology 24:88.
The specific activity is >20 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

4277-AS
Formulation Supplied as a 0.2 μm filtered solution in MES and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Renin

Renin is a aspartyl protease that plays a crucial role in the regulation of blood pressure and salt balance through the renin-angiotensin system (1). It cleaves angiotensinogen to generate angiotensin I, which can be further converted by angiotensin converting enzyme (ACE) to angiotensin II. Angiotensin II is the active molecule of the renin-angiotensin system that acts by binding to angiotensin receptors type 1 and 2 (AT-1 and AT-2), and has direct pathophysiological effects on the heart and peripheral vasculature (2). After secretion, inactive prorenin can be proteolytically activated by trypsin, cathepsin B, or other proteinases. Because of its unique specificity, inhibition of Renin is widely studied to provide selective therapy for hypertension, congestive heart failure and associated disorders (3).

References

  1. Fuminaki, S. et al. 2004 in Handbook of Proteolytic Enzymes, Barrett, A. J. et al. eds:54.
  2. Unger, T. et al. 2008 Medscape J. Med. 10:S4.
  3. Dhanaraj, V. et al. 1992 Nature 357:466.

Alternate Names

Angiotensinogenase, REN

Entrez Gene IDs

5972 (Human); 19701 (Mouse)

Gene Symbol

REN

UniProt

P06281

Additional Renin Products

Product Documents for Recombinant Mouse Renin Protein, CF

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Product Specific Notices for Recombinant Mouse Renin Protein, CF

QXL is a trademark of AnaSpec, Inc.

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