Recombinant Mouse ROR1 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9910-RO

R&D Systems, part of Bio-Techne
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9910-RO-050
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Key Product Details

Source

NS0

Accession #

Q9Z139

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all ROR1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse ROR1 protein
Mouse ROR1
(Gln30-Glu403)
Accession # Q9Z139		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln30 inferred from enzymatic pyroglutamate treatment revealing Glu31.

Predicted Molecular Mass

69 kDa

SDS-PAGE

88-100 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Mouse ROR1 Fc Chimera binds Biotinylated Recombinant Human/Mouse Wnt-5a (Catalog # BT645) with and ED50 of
10-60 µg/mL.

Scientific Data Images for Recombinant Mouse ROR1 Fc Chimera Protein, CF

Recombinant Mouse ROR1 Fc Chimera Protein SDS-PAGE

Recombinant Mouse ROR1 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Mouse ROR1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 88-100 kDa and 180-200 kDa, respectively.

Formulation, Preparation and Storage

9910-RO
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: ROR1

ROR1 (Receptor tyrosine kinase-like orphan receptor 1), also known as neurotrophic tyrosine kinase receptor-related 1 (NTRKR1), is a member of the ROR family within receptor tyrosine kinases (RTK) superfamily. Two ROR family members (ROR1 and ROR2) have been identified and are characterized by the intracellular tyrosine kinase domains, highly related to those of the Trk-family receptor tyrosine kinases, and by the extracellular Frizzled-like cysteine-rich domains and kringle domains, which are common to receptors of the Wnt family members (1-3). Mouse ROR1 is a type I transmembrane protein with 937 amino acids (aa) in length. It contains a 29 aa signal sequence, a 377 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 510 aa cytoplasmic region. Mouse ROR1 shares 99%, 97% and 66% aa sequence identity with rat ROR1, human ROR1 and mouse ROR2 respectively (2). ROR1 has been shown to play crucial roles in developmental morphogenesis by acting as receptors or co-receptors to mediate Wnt5a-induced signaling (4). The bioactivity of ROR1 is measured by its ability to bind biotinylated recombinant mouse Wnt-5a in a functional ELISA.

References

  1. Aghebati-Maleki, L. et al. (2017) Biomed. Pharmacother. 88:814.
  2. Borcherding N. et al. (2014) Protein Cell. 5:496.
  3. Bainbridge T. et al. (2014) PLoS One. 9:e102695.
  4. Fukuda T. et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105:3047.

Long Name

Receptor Tyrosine Kinase-like Orphan Receptor 1

Alternate Names

NTRKR1

Entrez Gene IDs

4919 (Human); 26563 (Mouse)

Gene Symbol

ROR1

UniProt

Q9Z139

Additional ROR1 Products

Product Documents for Recombinant Mouse ROR1 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse ROR1 Fc Chimera Protein, CF

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